LATERAL FLORET 1 induced the three-florets spikelet in rice
LATERAL FLORET 1 induced the three-florets spikelet in rice
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侧花1诱导水稻三花小穗
DOI：
10.1073/pnas.1700504114
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发表时间：
2017-08
影响因子：
11.1
通讯作者：
He Guanghua
中科院分区：
文献类型：
--
作者：
Zhang Ting;Li Yunfeng;Ma Ling;Sang Xianchun;Ling Yinghua;Wang Yantong;Yu Peng;Zhuang Hui;Huang Junyang;Wang Nan;Zhao Fangming;Zhang Changwei;Yang Zhenglin;Fang Likui;He Guanghua
在谷类作物中，小穗小花数是影响每穗粒数进而影响产量的重要因素。在野生型水稻中，一个小穗产生一个可育小花。本研究鉴定了一个水稻侧小花功能获得突变体lf 1。在lf 1中，小穗在不育外稃的腋部发育出具有适当花器官特性的侧生小花，表明水稻小穗具有恢复祖先可能存在的“三花小穗”的潜力。因此，为三小花颖花假说提供了有力的证据，并为利用三小花颖花育种提高每穗粒数提供了前景。小穗是禾本科植物独特的花序结构。小穗发育和进化背后的分子机制还很不清楚。在这项研究中，一个显性水稻突变体，侧小花1（lf 1），其特征在于。在lf 1小穗中，侧生分生组织意外地被促进，并且通常可以开花成相对正常的小花。LF 1编码一种III类同源结构域-亮氨酸拉链（HD-ZIP III）蛋白，其突变位点位于miRNA 165/166靶序列中。LF 1和分生组织维持基因OSH 1在lf 1小穗的不育外稃原基腋部都有异位表达。OSH 1启动子可与LF 1蛋白直接结合。总的来说，这些结果表明，LF 1的突变诱导OSH 1的异位表达，这导致在侧分生组织的启动，产生侧小花在腋部的不育外稃。本研究为水稻“三小花小穗”假说提供了有力的证据。
Significance In cereal crops, the number of florets in a spikelet is an important factor affecting the grain number per panicle and then the grain yield. In wild-type rice, one spikelet produces one fertile floret. This study characterized a gain-of-function mutant lateral florets 1 (lf1) in rice. In lf1, the spikelet developed lateral florets with proper floral organ identities in the axil of the sterile lemma, showing that the rice spikelet has the potential to restore the “three-florets spikelet” which may have existed in ancestors. Therefore, it provides strong evidence supporting the three-florets spikelet hypothesis and presents a prospect for increasing grain number per panicle by breeding rice with three-floret spikelets. The spikelet is a unique inflorescence structure in grass. The molecular mechanisms behind the development and evolution of the spikelet are far from clear. In this study, a dominant rice mutant, lateral florets 1 (lf1), was characterized. In the lf1 spikelet, lateral floral meristems were promoted unexpectedly and could generally blossom into relatively normal florets. LF1 encoded a class III homeodomain-leucine zipper (HD-ZIP III) protein, and the site of mutation in lf1 was located in a putative miRNA165/166 target sequence. Ectopic expression of both LF1 and the meristem maintenance gene OSH1 was detected in the axil of the sterile lemma primordia of the lf1 spikelet. Furthermore, the promoter of OSH1 could be bound directly by LF1 protein. Collectively, these results indicate that the mutation of LF1 induces ectopic expression of OSH1, which results in the initiation of lateral meristems to generate lateral florets in the axil of the sterile lemma. This study thus offers strong evidence in support of the “three-florets spikelet” hypothesis in rice.
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影响因子：
4.5
作者：
Shi B;Zhang C;Tian C;Wang J;Wang Q;Xu T;Xu Y;Ohno C;Sablowski R;Heisler MG;Theres K;Wang Y;Jiao Y
通讯作者：
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DOI：
10.1111/j.1365-313x.2008.03710.x
发表时间：
2009-02
期刊：
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影响因子：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
11.4
作者：
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通讯作者：
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