Pollen tube in hazel grows intermittently: Role of Ca2+ and expression of auto-inhibited Ca2+ pump
Pollen tube in hazel grows intermittently: Role of Ca2+ and expression of auto-inhibited Ca2+ pump
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榛树花粉管间歇性生长：Ca 2 的作用和自抑制 Ca 2 泵的表达
DOI：
10.1016/j.scienta.2021.110032
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发表时间：
2021-05
影响因子：
4.3
通讯作者：
Cheng Yunqing
中科院分区：
文献类型：
--
作者：
Liu Jianfeng;Liu Jianying;Zhang Xingzheng;Wei Heng;Ren Jiahua;Peng Cheng;Cheng Yunqing
由于雌花开花时没有子房，榛子花粉管呈现出明显的间歇生长模式，这对每丛果数和产量有重要影响，但对这一过程的调控机制知之甚少。在田间试验中，Ca2+和Ca2+- atp酶激活剂5-氨基乙酰丙酸（ALA）的喷雾处理可以抑制雌花花序下降，增加果实产量，而ALA处理的效果与钙处理相反。在柱头后部和子房原始细胞层中观察到高度积累的Ca2+。在体外培养条件下，Ca2+和ALA对花粉萌发率和管长分别有促进和抑制作用。ALA处理降低了花粉管Ca2+浓度，不利于花粉管顶端Ca2+梯度的形成。榛子基因组编码17个Ca2+- atp酶，包括14个自抑制Ca2+- atp酶和3个er型Ca2+- atp酶。Hazel Ca2+- atp酶家族蛋白，包括11个常见的跨膜结构域，是高度保守的。qRT-PCR分析显示，17种Ca2+- atp酶中chaca1和checa2在花粉管中高表达。在0 ~ 1.3 mM Ca2+范围内，花粉管中Ca2+荧光强度chaca1的表达量同步上升，在1.3 mM Ca2+时达到最大值，在1.6 mM Ca2+时下降，这与花粉管长度和checa2的变化趋势分别一致和不一致。花粉管中Ca2+- atp酶活性分析表明，Ca2+和ALA处理均显著提高了花粉管中Ca2+- atp酶活性。研究结果表明，chaca1可能是榛树花粉萌发和花粉管发育的关键调控因子，为榛树独特的花粉管间歇性生长机制提供了新的认识。
Owing to the absence of the ovary when the female inflorescence blooms, hazel pollen tubes show an obvious intermittent growth pattern, which has an important impact on the number of fruits per cluster and yield, but little is known about the mechanisms governing this process. Spray treatments of Ca2+and Ca2+-ATPase activator 5-aminolevulinic acid (ALA) in field experiment showed that Ca2+treatments inhibited pistillate inflorescence drop and produced more fruits, while the effects of ALA treatments was just opposite to that of calcium treatments. Highly accumulated Ca2+was observed at the rear part of the stigma and in the ovary primordial cell layers. Underin vitroculture conditions, pollen germination ratio and tube length were promoted and inhibited by Ca2+and ALA, respectively. ALA treatment reduced the Ca2+concentration in pollen tube, which was not conducive to the formation of a tip-focused Ca2+gradient in pollen tube. The hazel genome encoded 17 Ca2+-ATPases, including 14 auto-inhibited Ca2+-ATPases and three ER-type Ca2+-ATPases. Hazel Ca2+-ATPase family proteins, including 11 common transmembrane domains, were highly conserved. Of 17 Ca2+-ATPases, qRT-PCR analysis showed thatChACA1andChECA2were highly expressed in pollen tubes. In the range of 0–1.3 mM Ca2+, Ca2+fluorescence intensity in pollen tubes, expression ofChACA1continued to rise simultaneously, and they reached their maximum at 1.3 mM Ca2+, then declined at 1.6 mM Ca2+, which was consistent and inconsistent with changing trends of pollen tube length andChECA2respectively. Analysis of enzyme activity in pollen tubes showed that Ca2+-ATPase activity was promoted by both Ca2+and ALA treatments significantly. Our findings suggested thatChACA1may act as a key regulator of pollen germination and pollen tube development in hazel, and provide new insight into the mechanisms of unique pollen tube intermittent growth in hazel.
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影响因子：
4.8
作者：
Harper, JF;Hong, BM;Sze, H
通讯作者：
Sze, H
DOI：
10.1111/pce.12559
发表时间：
2015-11
期刊：
Plant, cell & environment
影响因子：
--
作者：
Shuang-Shuang Wang-Shuang;Wenzhu Diao;Xue Yang;Zhu Qiao;Meirong Wang;B. Acharya;Wei Zhang
通讯作者：
Shuang-Shuang Wang-Shuang;Wenzhu Diao;Xue Yang;Zhu Qiao;Meirong Wang;B. Acharya;Wei Zhang
通过比较转录组分析鉴定榛子授粉后调节子房分化的基因
影响因子：
5.3
作者：
Cheng Y;Zhang Y;Liu C;Ai P;Liu J
通讯作者：
Liu J
影响因子：
6.1
作者：
Amaral, JS;Casal, S;Oliveira, BPP
通讯作者：
Oliveira, BPP
影响因子：
4.3
作者：
Al-Qurashi, Adel D.;Awad, Mohamed A.
通讯作者：
Awad, Mohamed A.