Highly photostable nanogels for fluorescence-based theranostics.
Highly photostable nanogels for fluorescence-based theranostics.
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DOI：
10.1016/j.bioactmat.2017.03.001
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发表时间：
2018-03
影响因子：
18.9
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Gyawali D;Kim JP;Yang J
以可生物降解的聚合物为模板，制备了一种新型的光交联纳米凝胶，该凝胶具有固有的光致发光特性和高的光稳定性。荧光纳米凝胶显示出优异的生物降解性和细胞相容性，这是由于涉及无溶剂和无表面活性剂的一锅反应的简易合成方案，完全来自生物相容性单体柠檬酸，马来酸，L-半胱氨酸和聚（乙二醇）。所得纳米凝胶的直径小于200 nm，具有窄的尺寸分布和单分散性，并在生物缓冲液中表现出两周的长期结构稳定性。为了测量治疗诊断应用中的潜力，荧光纳米凝胶用生物活性RGD肽进行表面功能化，并用活性抗癌药物阿霉素包封，从而在类似肿瘤环境的酸性pH中产生pH响应性受控药物释放。纳米凝胶的强荧光使得能够跟踪靶向药物递送，表明载药纳米凝胶归巢到前列腺癌细胞的细胞质区域以显著诱导细胞死亡。这些可光交联和可生物降解的纳米凝胶成为治疗诊断医学的强有力候选者，表现出多功能化，在生物缓冲液中的高稳定性，以及基于荧光的靶向药物递送实时监测的能力。用于细胞标记的可光交联的基于柠檬酸盐的生物可降解聚合物纳米凝胶。通过柠檬酸合成法制备了具有本征光致发光的可光交联纳米凝胶。该纳米凝胶具有良好的光稳定性、生物降解性和细胞相容性。纳米凝胶表面功能化的RGD肽和阿霉素负载，使治疗诊断药物输送。
A novel photo-crosslinkable nanogel is prepared from a biodegradable polymer template with intrinsic photoluminescence and high photostability. The fluorescent nanogels display excellent biodegradability and cytocompatibility owed to the facile synthesis scheme involving a solvent- and surfactant-free one-pot reaction, derived entirely from biocompatible monomers citric acid, maleic acid, L-cysteine, and poly(ethylene glycol). The resultant nanogels are less than 200 nm in diameter with a narrow size distribution and monodispersity, and demonstrate long-term structural stability in biological buffer for two weeks. To gauge potential in theranostic applications, the fluorescent nanogels were surface functionalized with biologically active RGD peptides and encapsulated with active anti-cancer drug Doxorubicin, resulting in a pH-responsive controlled drug release in acidic pH resembling tumor environments. The strong fluorescence of the nanogels enabled tracking of targeted drug delivery, showing that drug-loaded nanogels homed into the cytoplasmic regions of prostate cancer cells to significantly induce cell death. These photo-crosslinkable and biodegradable nanogels pose as a strong candidate for theranostic medicine, demonstrating versatile functionalization, high stability in biological buffers, and capacity for real-time fluorescence-based monitoring of targeted drug delivery. Photo-crosslinkable citrate-based biodegradable polymeric nanogels for cell labeling. A photo-crosslinkable nanogel with intrinsic photoluminescence is prepared from a facile citric acid-based synthesis. The nanogels displayed high photostability, excellent biodegradability, and cytocompatibility. Nanogels were surface functionalized with RGD peptides and loaded with Doxorubicin, enabling theranostic drug delivery.
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影响因子：
8.4
作者：
Kim JP;Xie Z;Creer M;Liu Z;Yang J
通讯作者：
Yang J
影响因子：
3.8
作者：
Dickerson EB;Blackburn WH;Smith MH;Kapa LB;Lyon LA;McDonald JF
通讯作者：
McDonald JF
影响因子：
10
作者：
Gyawali, Dipendra;Zhou, Shengyuan;Tran, Richard T.;Zhang, Yi;Liu, Chao;Bai, Xiaochun;Yang, Jian
通讯作者：
Yang, Jian
影响因子：
14
作者：
Zhang, Yi;Tran, Richard T.;Qattan, Ibrahim S.;Tsai, Yi-Ting;Tang, Liping;Liu, Chao;Yang, Jian
通讯作者：
Yang, Jian
影响因子：
9.7
作者：
Tran RT;Yang J;Ameer GA
通讯作者：
Ameer GA