A Monoclonal Antibody Against the PSTAIR Sequence of p34cdc2, Catalytic Subunit of Maturation‐Promoting Factor and Key Regulator of the Cell Cycle
A Monoclonal Antibody Against the PSTAIR Sequence of p34cdc2, Catalytic Subunit of Maturation‐Promoting Factor and Key Regulator of the Cell Cycle
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针对 p34cdc2 PSTAIR 序列的单克隆抗体，p34cdc2 是成熟促进因子的催化亚基和细胞周期的关键调节因子
DOI：
10.1111/j.1440-169x.1991.00617.x
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发表时间：
1991
期刊：
影响因子：
4.6
通讯作者：
Y. Nagahama
中科院分区：
文献类型：
--
作者：
M. Yamashita;M. Yoshikuni;T. Hirai;S. Fukada;Y. Nagahama
丝氨酸/苏氨酸蛋白激酶（p34 cdc 2）的同源物，由裂殖酵母（裂殖酵母）的cdc 2+基因编码，是成熟促进因子的催化亚基和细胞周期的关键调节因子。我们已经提出了一个单克隆抗体对最保守的氨基酸序列，PSTAIR序列（EGVPSTAIREISLLKE）的p34 cdc 2该抗体识别31-34 kDa的蛋白质，通过免疫印迹在所有物种的检查到目前为止。抗PSTAIR抗体识别的蛋白质可能是p34 cdc 2本身或与给定物种中的p34 cdc 2高度同源的蛋白质，因为在迄今为止的所有物种研究中，它们都与p13 suc 1沉淀，p13 suc 1是裂殖酵母suc 1+基因产物，其以高特异性结合p34 cdc 2。抗PSTAIR免疫沉淀物没有组蛋白H1激酶活性，也不含细胞周期蛋白B，这表明当p34 cdc 2与作为活性激酶的细胞周期蛋白B形成复合物时，PSTAIR区域被掩蔽。用抗PSTAIR抗体进行的免疫印迹表明，当卵母细胞成熟或细胞进入M期时，p34 cdc 2同源物的最快迁移形式变得丰富。这一观察结果的可能意义与p34 cdc 2的磷酸化和活性状态有关。在各种物种中观察到的抗PSTAIR抗体对p34 cdc 2同源物的广泛交叉反应性应允许我们检查p34 cdc 2同源物在各种生物体中调节细胞周期的作用。
A homolog of the serine/threonine protein kinase (p34cdc2), encoded by the cdc2+ gene of the fission yeast (Schizosaccharomyces pombe), is a catalytic subunit of maturation‐promoting factor and a key regulator of the cell cycle. We have raised a monoclonal antibody against the most conserved amino acid sequence, the PSTAIR sequence (EGVPSTAIREISLLKE) of p34cdc2 This antibody recognizes 31–34 kDa proteins by immunoblotting in all species examined so far. The proteins recognized by the anti‐PSTAIR antibody are probably either p34cdc2 itself or proteins highly homologous to p34cdc2 in the given species, since, in all species studies to date, they are all precipitated with p13suc1, the fission yeast suc1+ gene product, which binds to p34cdc2 with high specificity. The anti‐PSTAIR immunoprecipitate had no histone H1 kinase activity and did not contain cyclin B, suggesting that the PSTAIR region is masked when p34cdc2 forms a complex with cyclin B as an active kinase. Immunoblotting with the anti‐PSTAIR antibody demonstrated that the fastest‐migrating form of p34cdc2 homologues becomes abundant, when oocytes mature or the cell enters M phase. The possible significance of this observation is discussed in relation to the phosphorylation and activity state of p34cdc2 The observed broad cross‐reactivity of the anti‐PSTAIR antibody against p34cdc2 homologues in various species should permit us to examine the role of p34cdc2 homologues in the regulation of the cell cycle in a variety of organisms.
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通过修饰 p34cdc2 和细胞周期蛋白成分的磷酸化来激活 M 期特异性组蛋白 H1 激酶。
影响因子：
10.5
作者：
Pondaven,P;Meijer,L;Beach,D
通讯作者：
Beach,D
DOI：
10.1073/pnas.86.16.6255
发表时间：
1989-08-01
影响因子：
11.1
作者：
HADWIGER, JA;WITTENBERG, C;REED, SI
通讯作者：
REED, SI
DOI：
10.1073/pnas.85.9.3009
发表时间：
1988-05-01
影响因子：
11.1
作者：
LOHKA, MJ;HAYES, MK;MALLER, JL
通讯作者：
MALLER, JL
影响因子：
11.4
作者：
BRIZUELA, L;DRAETTA, G;BEACH, D
通讯作者：
BEACH, D