Increase in fragmented phosphatidylcholine in blood plasma by oxidative stress.
Increase in fragmented phosphatidylcholine in blood plasma by oxidative stress.
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氧化应激导致血浆中碎片磷脂酰胆碱增加。
DOI：
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发表时间：
2000
影响因子：
6.5
通讯作者：
M. Schlame
中科院分区：
文献类型：
--
作者：
B. Frey;R. Haupt;Sonja Alms;G. Holzmann;T. König;H. Kern;W. Kox;B. Rüstow;M. Schlame
氧化修饰的磷脂与断裂的酰基链已经吸引了很大的兴趣，因为它们的促炎活性和它们的潜在参与动脉粥样硬化。它们可以通过不饱和磷脂的自由基处理在体外形成，但尚不清楚它们在体内积累的条件。本文报道了用氯甲基蒽柱前衍生化高效液相色谱法测定人血浆中一种片段磷脂酰胆碱（PC）的含量。结构分析表明，片段PC是一个二酰基物种与棕榈酰基和短的氧化残基，其中最有可能有四个碳。老年冠心病患者的碎片PC浓度高于年轻健康对照组。吸烟一根香烟急剧增加了健康成年人中碎片PC的浓度。在体外循环治疗后的再灌注期，碎片PC也增加。这种增加与循环中性粒细胞的激增相吻合。在大鼠中，碎片PC的血浆浓度升高，维生素E缺乏和暴露于高氧。数据表明，碎片PC增加血浆中的各种形式的氧化应激反应。
Oxidatively modified phospholipids with fragmented acyl chains have attracted much interest because of their proinflammatory activity and their potential involvement in atherosclerosis. They can be formed in vitro by free radical treatment of unsaturated phospholipids but it is not known under which conditions they accumulate in vivo. We assayed one species of fragmented phosphatidylcholine (PC) in human blood plasma by high performance liquid chromatography after precolumn derivatization with chloromethylanthracene. Structural analysis suggested that fragmented PC was a diacyl species with a palmitoyl group and a short oxidized residue, which most likely had four carbons. The concentration of fragmented PC was higher in elderly individuals with coronary heart disease than in young healthy controls. Smoking one cigarette acutely increased the concentration of fragmented PC in healthy adults. Fragmented PC also increased in the reperfusion period after treatment with cardiopulmonary bypass. The increase coincided with a surge of circulating neutrophils. In rats, the plasma concentration of fragmented PC was elevated by vitamin E deficiency and exposure to high oxygen. The data demonstrate that fragmented PC increases in blood plasma in response to various forms of oxidative stress.
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