Analysis of GABA-induced inhibition of spontaneous firing in chick accessory lobe neurons
Analysis of GABA-induced inhibition of spontaneous firing in chick accessory lobe neurons
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GABA 诱导的鸡副叶神经元自发放电抑制分析
DOI：
10.1007/s00359-011-0703-0
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Izumi Shibuya
中科院分区：
文献类型：
--
作者：
Yuko Yamanaka;Naoki Kitamura;Hikaru Shinohara;Keita Takahashi;Izumi Shibuya
鸡的副叶（AL）中含有功能性神经元，是一种平衡的感觉器官。据报道，位于AL外层的神经元显示γ-氨基丁酸（GABA）和谷氨酸脱羧酶（GAD）样免疫反应比位于中央的神经元更强，中央神经元被GAD免疫反应阳性终末包围。我们研究了GABA对AL神经元电活动的影响。约50%的胚胎AL神经元表现出自发放电。在细胞上记录中，GABA、蝇蕈醇和GABA与CGP 35348组合抑制这种放电。在全细胞电压钳记录中，GABA和蝇蕈醇诱发了瞬时电流。GABA诱发电流的平均反转电位接近Cl−的理论反转电位。这些结果表明，GABA通过激活GABAA受体对放电产生抑制作用。此外，在短杆菌肽穿孔配置的测量中，细胞内Cl−的浓度估计为约16 mM，表明GABA电流的生理逆转电位约为−60 mV。总之，AL神经元具有诱发自发放电的内在机制，其自发放电可通过激活GABA A受体的抑制机制而被抑制。
It has been hypothesized that chick accessory lobes (ALs) contain functional neurons and act as a sensory organ of equilibrium. It was reported that neurons located in an outer layer of ALs showed γ-aminobutyric acid (GABA)- and glutamic acid decarboxylase (GAD)-like immunoreactivity more strongly than centrally located neurons, which were surrounded by the GAD-immunoreactive terminals. We investigated effects of GABA on the electrical activity of AL neurons. About 50% of embryonic AL neurons exhibited spontaneous firing. In the on-cell recording, GABA, muscimol, and GABA in combination with CGP35348 inhibited this firing. In whole-cell voltage clamp recordings, GABA and muscimol evoked a transient current. The mean reversal potential of GABA-evoked currents was close to the theoretical reversal potential of Cl−. These results indicate that GABA exerts the inhibitory effect on the firing through the activation of GABAAreceptors. In addition, the intracellular concentration of Cl−was estimated to be about 16 mM in measurements with the gramicidin-perforated configuration, indicating the physiological reversal potential of the GABA current was about −60 mV. In conclusion, AL neurons have an intrinsic mechanism to evoke the spontaneous firing, which can be arrested by the inhibitory mechanism through the activation of the GABAAreceptors.
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DOI：
10.1007/s00429-005-0016-6
发表时间：
2005-08-01
期刊：
ANATOMY AND EMBRYOLOGY
影响因子：
--
作者：
Necker, R
通讯作者：
Necker, R
鸽子脊髓边缘核的组织化学和免疫细胞化学研究（Columba livia）
影响因子：
3.8
作者：
Torsten Milinski;R. Necker
通讯作者：
R. Necker
影响因子：
2.5
作者：
GOLDBERG, JM;FERNANDEZ, C
通讯作者：
FERNANDEZ, C
松鼠猴半规管周围神经元的生理学。
影响因子：
2.5
作者：
J. Goldberg;C. Fernández
通讯作者：
C. Fernández
DOI：
10.1007/s003590050440
发表时间：
2000-04-01
期刊：
JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY
影响因子：
--
作者：
Necker, R;Janssen, A;Beissenhirtz, T
通讯作者：
Beissenhirtz, T