A Systemic and Local Comparison of Senescence in an Acute Anterior Cruciate Ligament Injury-A Pilot Case Series.
A Systemic and Local Comparison of Senescence in an Acute Anterior Cruciate Ligament Injury-A Pilot Case Series.
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DOI：
10.3390/life13071567
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发表时间：
2023-07-15
期刊：
影响因子：
3.2
通讯作者：
Huard, Johnny
中科院分区：
文献类型：
--
作者：
Waltz, Robert A. A.;Whitney, Kaitlyn E. E.;Duke, Victoria R. R.;Kloser, Heidi;Huard, Charles;Provencher, Matthew T. T.;Philippon, Marc J. J.;Bahney, Chelsea;Godin, Jonathan A. A.;Huard, Johnny
关键词：
背景：衰老是细胞衰老和炎症的一个特征，与骨关节炎的加速有关。本研究的目的是前瞻性地识别、测量和比较急性膝关节损伤患者 48 小时内滑液和外周血的衰老特征。方法：前瞻性纳入 7 名年龄 18-60 岁、患有急性 ACL 撕裂并渗出的受试者。收集滑液和外周血样本，并使用衰老标记物 C12FDG 和 CD87 通过流式细胞术进行分析。使用 qRT-PCR 和多重免疫测定在基因和蛋白质水平上探讨衰老表型与促再生表型。结果：所有患者的滑液和外周血中均检测到C12FDG和CD87阳性衰老细胞。滑液中测量的促炎性 IL-1β 基因表达显着高于全身/血液表达（p = 0.0156）。与血清相比，滑液中的衰老相关因子 MMP-3 和再生因子 TIMP-2 显着升高。与滑液相比，血清中衰老相关因子 MMP-9 和再生因子 TGFβ-2 显着升高。相关分析显示，滑液中的C12FDG++/CD87++衰老细胞与年龄相关生长调节癌基因（ρ = 1.00，p < 0.001）、IFNγ（ρ = 1.00，p < 0.001）、IL-8（ρ = 0.90，p = 0.0374）和基因标记物p16（ρ = 0.83，p = 0.0416）。结论：急性 ACL 撕裂后，局部和全身都有大量的衰老细胞，外周血中的衰老细胞与滑液中的衰老细胞没有显着差异。这些初步数据可能有助于确定在急性韧带损伤或重建手术时改变滑液内急性环境的策略。
Background: Senescence, a characteristic of cellular aging and inflammation, has been linked to the acceleration of osteoarthritis. The purpose of this study is to prospectively identify, measure, and compare senescent profiles in synovial fluid and peripheral blood in patients with an acute knee injury within 48 h. Methods: Seven subjects, aged 18–60 years, with an acute ACL tear with effusion were prospectively enrolled. Synovial fluid and peripheral blood samples were collected and analyzed by flow cytometry, using senescent markers C12FDG and CD87. The senescent versus pro-regenerative phenotype was probed at a gene and protein level using qRT-PCR and multiplex immunoassays. Results: C12FDG and CD87 positive senescent cells were detected in the synovial fluid and peripheral blood of all patients. Pro-inflammatory IL-1β gene expression measured in synovial fluid was significantly higher (p = 0.0156) than systemic/blood expression. Senescent-associated factor MMP-3 and regenerative factor TIMP-2 were significantly higher in synovial fluid compared to blood serum. Senescent-associated factor MMP-9 and regenerative factor TGFβ-2 were significantly elevated in serum compared to synovial fluid. Correlation analysis revealed that C12FDG++/CD87++ senescent cells in synovial fluid positively correlated with age-related growth-regulated-oncogene (ρ = 1.00, p < 0.001), IFNγ (ρ = 1.00, p < 0.001), IL-8 (ρ = 0.90, p = 0.0374), and gene marker p16 (ρ = 0.83, p = 0.0416). Conclusions: There is an abundance of senescent cells locally and systemically after an acute ACL tear without a significant difference between those present in peripheral blood compared to synovial fluid. This preliminary data may have a role in identifying strategies to modify the acute environment within the synovial fluid, either at the time of acute ligament injury or reconstruction surgery.
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影响因子：
4.8
作者：
Chatfield, Mark;Mander, Adrian
通讯作者：
Mander, Adrian
影响因子：
4.8
作者：
Clair, Andrew J.;Kingery, Matthew T.;Strauss, Eric J.
通讯作者：
Strauss, Eric J.
影响因子：
2.8
作者：
Maerz, Tristan;Sherman, Eric;Baker, Kevin C.
通讯作者：
Baker, Kevin C.
DOI：
10.1146/annurev-pathol-121808-102144
发表时间：
2010
期刊：
Annual review of pathology
影响因子：
--
作者：
Coppé JP;Desprez PY;Krtolica A;Campisi J
通讯作者：
Campisi J
影响因子：
7
作者：
Heard, B. J.;Solbak, N. M.;Frank, C. B.
通讯作者：
Frank, C. B.