Current status and future prospects of an automated sample exchange system PAM for protein crystallography
Current status and future prospects of an automated sample exchange system PAM for protein crystallography
复制标题
蛋白质晶体学自动样品交换系统PAM的现状与未来展望
DOI：
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
S. Wakatsuki
中科院分区：
文献类型：
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作者：
M. Hiraki;Yusuke Yamada;L. Chavas;N. Matsugaki;Noriyuki Igarashi;S. Wakatsuki
为了在高通量X射线实验中实现全自动和/或远程数据收集，光子工厂（PF）的结构生物学研究中心在PF大分子晶体学光束线BL-1A，BL-5A，BL-17 A，AR-NW 12 A和AR-NE 3A上安装了PF自动安装系统（PAM）用于样品交换机器人。我们正在升级实验系统，包括PAM，以实现稳定和高效的操作。为了防止自动数据收集中的人为错误，我们安装了一个二维条形码阅读器，用于识别试剂卡和样本针。由于PF实验箱内没有安装液氮管路，用户一般用小型杜瓦瓶加注液氮。为了解决这个问题，一个自动化的液氮填充系统，连接一个100升的坦克到机器人杜瓦已安装在PF高分子束线。在这里，我们描述了这种新的实现，以及未来的前景。
To achieve fully-automated and/or remote data collection in high-throughput X-ray experiments, the Structural Biology Research Centre at the Photon Factory (PF) has installed PF automated mounting system (PAM) for sample exchange robots at PF macromolecular crystallography beamlines BL-1A, BL-5A, BL-17A, AR-NW12A and AR-NE3A. We are upgrading the experimental systems, including the PAM for stable and efficient operation. To prevent human error in automated data collection, we installed a two-dimensional barcode reader for identification of the cassettes and sample pins. Because no liquid nitrogen pipeline in the PF experimental hutch is installed, the users commonly add liquid nitrogen using a small Dewar. To address this issue, an automated liquid nitrogen filling system that links a 100-liter tank to the robot Dewar has been installed on the PF macromolecular beamline. Here we describe this new implementation, as well as future prospects.
影响因子：
6.1
作者：
Cohen, AE;Ellis, PJ;Phizackerley, RP
通讯作者：
Phizackerley, RP