The genetic basis of variation in clean lineages of Saccharomyces cerevisiae in response to stresses encountered during bioethanol fermentations.
The genetic basis of variation in clean lineages of Saccharomyces cerevisiae in response to stresses encountered during bioethanol fermentations.
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DOI：
10.1371/journal.pone.0103233
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Smart KA
中科院分区：
文献类型：
--
作者：
Greetham D;Wimalasena TT;Leung K;Marvin ME;Chandelia Y;Hart AJ;Phister TG;Tucker GA;Louis EJ;Smart KA
酿酒酵母是将单糖转化为生物乙醇的首选微生物。工业生物乙醇发酵本身就是酵母的应激环境，其适应性保护反应因菌株背景而异。为了确定调控表型变异的数量性状位点（QTL），对酿酒酵母4个高度分化洁净系的6个F1杂交进行了连锁分析。每个杂交的分离体对工业生物乙醇发酵过程中遇到的一系列压力的耐受性进行了评估。不同群体对胁迫条件的耐受性存在差异，表现为越界变异。连锁分析鉴定了耐弱酸和渗透胁迫的QTL。我们使用互易半合子分析对QTL鉴定位点内的候选基因进行测试，以确定它们对观察到的表型变异的贡献；这种方法验证了一个基因（COX20）对弱酸性胁迫和一个基因（RCK2）对渗透胁迫。具有敏感表型的半合子转化子携带来自弱酸敏感亲本的COX20等位基因，与其他酿酒葡萄球菌菌株相比，其蛋白质编码发生了改变。RCK2等位基因揭示了亲本菌株之间的肽差异，这些变化的重要性目前正在确定。
Saccharomyces cerevisiae is the micro-organism of choice for the conversion of monomeric sugars into bioethanol. Industrial bioethanol fermentations are intrinsically stressful environments for yeast and the adaptive protective response varies between strain backgrounds. With the aim of identifying quantitative trait loci (QTL's) that regulate phenotypic variation, linkage analysis on six F1 crosses from four highly divergent clean lineages of S. cerevisiae was performed. Segregants from each cross were assessed for tolerance to a range of stresses encountered during industrial bioethanol fermentations. Tolerance levels within populations of F1 segregants to stress conditions differed and displayed transgressive variation. Linkage analysis resulted in the identification of QTL's for tolerance to weak acid and osmotic stress. We tested candidate genes within loci identified by QTL using reciprocal hemizygosity analysis to ascertain their contribution to the observed phenotypic variation; this approach validated a gene (COX20) for weak acid stress and a gene (RCK2) for osmotic stress. Hemizygous transformants with a sensitive phenotype carried a COX20 allele from a weak acid sensitive parent with an alteration in its protein coding compared with other S. cerevisiae strains. RCK2 alleles reveal peptide differences between parental strains and the importance of these changes is currently being ascertained.
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影响因子：
14.8
作者：
Gietz, R. Daniel;Schiestl, Robert H.
通讯作者：
Schiestl, Robert H.
影响因子：
5
作者：
Fernandez-Sandoval, M. T.;Huerta-Beristain, G.;Martinez, A.
通讯作者：
Martinez, A.
DOI：
10.1534/g3.111.000422
发表时间：
2011-09
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Ambroset C;Petit M;Brion C;Sanchez I;Delobel P;Guérin C;Chiapello H;Nicolas P;Bigey F;Dequin S;Blondin B
通讯作者：
Blondin B
影响因子：
4.4
作者：
Aslankoohi, Elham;Zhu, Bo;Verstrepen, Kevin J.
通讯作者：
Verstrepen, Kevin J.
影响因子：
7
作者：
Parts, Leopold;Cubillos, Francisco A.;Liti, Gianni
通讯作者：
Liti, Gianni