Are sub-2 μm particles best for separating small molecules? An alternative.
Are sub-2 μm particles best for separating small molecules? An alternative.
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DOI：
10.1016/j.chroma.2014.09.078
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发表时间：
2014-11-14
期刊：
影响因子：
--
通讯作者：
Kirkland JJ
中科院分区：
文献类型：
--
作者：
DeStefano JJ;Boyes BE;Schuster SA;Miles WL;Kirkland JJ
2.5 - 2.7 μm范围内的表面多孔颗粒（SPP）提供与亚2 μm全多孔颗粒（TPP）几乎相同的效率和分辨率，但工作压力为二分之一到三分之一。SPP的优势导致了亚2 μm SPP的引入，作为该技术的自然扩展。虽然SPP和TPP亚2 μm颗粒的短柱允许非常快速的分离，但这些非常小的颗粒的效率优势通常没有实现，也不足以克服这些小颗粒的一些实际限制和缺点。描述了用小于2 μm的颗粒填充的柱的优点和缺点，以与较大颗粒的特性进行比较。作者得出结论，虽然亚2 μm颗粒在研究中具有实用性，但较大颗粒的柱通常更适合大多数应用。建议的2.0 μm表面多孔颗粒直径保留了亚2 μm颗粒的许多优点，但最大限度地减少了一些缺点。这些新型2.0 μm SPP的特征在比较目前一些亚2 μm SPP商业色谱柱的效率、柱床均匀性和稳定性的研究中进行了描述。
Superficially porous particles (SPP) in the 2.5 - 2.7 μm range provide almost the same efficiency and resolution of sub-2 μm totally porous particles (TPP), but at one-half to one-third of the operating pressure. The advantage of SPP has led to the introduction of sub-2 μm SPP as a natural extension of this technology. While short columns of both SPP and TPP sub-2 μm particles allow very fast separations, the efficiency advantages of these very small particles often are not realized nor sufficient to overcome some of the practical limitations and disadvantages of such small particles. Advantages and disadvantages of columns packed with sub-2 μm particles are described for comparison with the characteristics of larger particles. The authors conclude that while sub-2 μm particles have utility in research studies, columns of larger particles are often better suited for most applications. A suggested 2.0 μm superficially porous particle diameter retains many of the advantages of sub-2 μm particles, but minimizes some of the disadvantages. The characteristics of these new 2.0 μm SPP are described in studies comparing some present sub-2 μm SPP commercial columns for efficiency, column bed homogeneity and stability.
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影响因子：
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