Long-term oral administration of naringenin counteracts aging-related retinal degeneration via regulation of mitochondrial dynamics and autophagy.
Long-term oral administration of naringenin counteracts aging-related retinal degeneration via regulation of mitochondrial dynamics and autophagy.
复制标题
长期口服柚皮素可通过调节线粒体动力学和自噬来对抗衰老相关的视网膜变性
DOI：
10.3389/fphar.2022.919905
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发表时间：
2022
影响因子：
5.6
通讯作者：
Zhang, Xu
中科院分区：
文献类型：
--
作者：
Chen, Guiping;Zeng, Ling;Yan, Feng;Liu, Jinlong;Qin, Mengqi;Wang, Feifei;Zhang, Xu
衰老相关的视网膜变性可表现为由于视网膜结构损伤和视网膜稳态功能障碍而导致的视觉功能下降。柚皮素是一种黄酮类化合物，在预防细胞衰老、保护光感受器功能和减缓视觉功能丧失方面具有有益作用。然而，柚皮素在衰老小鼠视网膜中的作用和潜在机制需要进一步研究。本研究采用视网膜电图（ERG）和苏木精-伊红染色观察柚皮素对衰老眼的影响，并通过免疫印迹法探讨其可能的作用机制。视网膜电图显示，柚皮素使衰老小鼠视网膜暗视3.0、10.0的a波和b波振幅以及明视3.0的a波振幅增加。此外，施用柚皮素防止了总视网膜、神经节细胞、内丛状层、内核层和外核层中的老化诱导的视网膜变性。与溶剂组相比，柚皮素组线粒体融合蛋白2表达增加，OPA 1蛋白表达和L-OPA 1/S-OPA 1比值不变，动力蛋白相关蛋白1表达降低。此外，通过用柚皮素治疗，衰老小鼠中自噬中与年龄相关的改变的下调被显著挽救。总之，这些结果表明，口服施用柚皮素改善了老化小鼠视网膜中的视觉功能、视网膜结构、线粒体动力学和自噬。柚皮素可能是一种潜在的膳食补充剂，用于预防或治疗与衰老相关的视网膜变性。
Aging-related retinal degeneration can manifest as decreased visual function due to damage to retinal structures and dysfunction in retinal homeostasis. Naringenin, a flavonoid, has beneficial effects in preventing cellular aging, preserving the functionality of photoreceptors, and slowing down visual function loss. However, the role and potential mechanism of naringenin in the aging mouse retina require further investigation. In this study, we evaluated the effects of naringenin on the aging eye using electroretinogram (ERG) and hematoxylin and eosin staining and explored its potential mechanism by western blotting. ERG showed that naringenin increased the amplitude of the a- and b-waves of scotopic 3.0, 10.0, and the a-wave amplitude of photopic 3.0 in the aging mouse retina. Furthermore, administration of naringenin prevented aging-induced retinal degeneration in the total retina, ganglion cell, inner plexiform layer, inner nuclear layer, and outer nuclear layer. The expression of mitochondrial fusion protein two was increased, OPA1 protein expression and the ratio of L-OPA1/S-OPA1 were unchanged, and dynamin-related protein one was decreased in the 12-month-old mice treated with naringenin compared with the 12-month-old mice treated with vehicle. Furthermore, the downregulation of age-related alterations in autophagy was significantly rescued in the aging mice by treatment with naringenin. Taken together, these results suggest that the oral administration of naringenin improves visual function, retinal structure, mitochondrial dynamics, and autophagy in the aging mouse retinas. Naringenin may be a potential dietary supplement for the prevention or treatment of aging-related retinal degeneration.
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影响因子：
13.3
作者：
Baek, Ahruem;Son, Sumin;Kim, Dong-Eun
通讯作者：
Kim, Dong-Eun
影响因子：
5
作者：
Lin JB;Tsubota K;Apte RS
通讯作者：
Apte RS
影响因子：
8.8
作者：
Aparicio, Ricardo;Rana, Anil;Walker, David W.
通讯作者：
Walker, David W.
影响因子：
29
作者：
Mills KF;Yoshida S;Stein LR;Grozio A;Kubota S;Sasaki Y;Redpath P;Migaud ME;Apte RS;Uchida K;Yoshino J;Imai SI
通讯作者：
Imai SI
影响因子：
5.9
作者：
Piano I;Di Paolo M;Corsi F;Piragine E;Bisti S;Gargini C;Di Marco S
通讯作者：
Di Marco S