Shootin1: A protein involved in the organization of an asymmetric signal for neuronal polarization.
Shootin1: A protein involved in the organization of an asymmetric signal for neuronal polarization.
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DOI：
10.1083/jcb.200604160
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发表时间：
2006-10-09
期刊：
影响因子：
--
通讯作者：
Inagaki N
中科院分区：
文献类型：
--
作者：
Toriyama M;Shimada T;Kim KB;Mitsuba M;Nomura E;Katsuta K;Sakumura Y;Roepstorff P;Inagaki N
即使在对称的体外环境中，神经元也具有非凡的能力。虽然最近的研究表明，不对称的细胞内信号可以诱导神经元极化，但仍然不清楚这些极化信号是如何组织没有不对称的线索。我们描述了一种新的蛋白质，命名为shootin 1，在海马神经元极化过程中上调，并开始在多个神经突之间波动积累。最终，shootin 1积累在一个单一的轴突，这导致轴突诱导极化不对称。通过过量shootin 1干扰shootin 1的不对称组织破坏极化，而抑制shootin 1表达抑制极化。过表达和RNA干扰的数据表明，shootin 1所需的空间定位磷酸肌醇-3-激酶活性。shootin 1顺行运输到生长锥和扩散回索马;抑制这种运输防止其在神经元中的不对称积累。我们建议，shootin 1参与神经元极化所需的内部不对称信号的产生。
Neurons have the remarkable ability to polarize even in symmetrical in vitro environments. Although recent studies have shown that asymmetric intracellular signals can induce neuronal polarization, it remains unclear how these polarized signals are organized without asymmetric cues. We describe a novel protein, named shootin1, that became up-regulated during polarization of hippocampal neurons and began fluctuating accumulation among multiple neurites. Eventually, shootin1 accumulated asymmetrically in a single neurite, which led to axon induction for polarization. Disturbing the asymmetric organization of shootin1 by excess shootin1 disrupted polarization, whereas repressing shootin1 expression inhibited polarization. Overexpression and RNA interference data suggest that shootin1 is required for spatially localized phosphoinositide-3-kinase activity. Shootin1 was transported anterogradely to the growth cones and diffused back to the soma; inhibiting this transport prevented its asymmetric accumulation in neurons. We propose that shootin1 is involved in the generation of internal asymmetric signals required for neuronal polarization.
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