Rhamnogalacturonan, a chemically-defined polysaccharide, improves intestinal barrier function in DSS-induced colitis in mice and human Caco-2 cells.
Rhamnogalacturonan, a chemically-defined polysaccharide, improves intestinal barrier function in DSS-induced colitis in mice and human Caco-2 cells.
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DOI：
10.1038/s41598-018-30526-2
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发表时间：
2018-08-16
影响因子：
4.6
通讯作者：
Baggio CH
中科院分区：
文献类型：
--
作者：
Maria-Ferreira D;Nascimento AM;Cipriani TR;Santana-Filho AP;Watanabe PDS;Sant Ana DMG;Luciano FB;Bocate KCP;van den Wijngaard RM;Werner MFP;Baggio CH
天然多糖因其有益的生物学效应而成为一类重要的生物活性化合物。本文研究了从大头菜Acmella holacea(L.)中分离得到的鼠李半乳糖醛酸苷(Rgal)的保护和愈合作用。R.K.Jansen在小鼠肠道炎症的实验模型和异种人上皮结直肠腺癌细胞(Caco-2)中留下了叶子。结果表明，RGal治疗7天可通过保护小鼠体重减轻、大体损伤和缩短结肠长度来减轻DSS诱导的结肠炎的严重程度。与DSS组相比，RGal在保护结肠上皮细胞的同时，还促进了粘膜上皮细胞和粘液分泌杯状细胞的维持，维持了胶原蛋白的稳态，促进了细胞的增殖。在体外屏障功能实验中，RGal降低了IL-1β作用后细胞的通透性，同时减少了IL-8的分泌和Claudin-1的表达，并维持了occludin的分布。此外，我们还观察到RGal对Caco-2上皮细胞系创面愈合的促进作用。综上所述，RGal在体内和体外均能改善肠屏障功能，有望成为治疗溃疡性结肠炎的一种有吸引力的分子。
Natural polysaccharides have emerged as an important class of bioactive compounds due their beneficial biological effects. Here we investigated the protective and healing effects of rhamnogalacturonan (RGal) isolated from Acmella oleracea (L.) R.K. Jansen leaves in an experimental model of intestinal inflammation in mice and in heterogeneous human epithelial colorectal adenocarcinoma cells (Caco-2). The findings demonstrated that RGal treatment for 7 days reduced the severity of DSS-induced colitis by protecting mice from weight loss, macroscopic damage and reduction of colon length. When compared to the DSS group, RGal also protected the colon epithelium and promoted the maintenance of mucosal enterocytes and mucus secreting goblet cells, in addition to conserving collagen homeostasis and increasing cell proliferation. In an in vitro barrier function assay, RGal reduced the cellular permeability after exposure to IL-1β, while decreasing IL-8 secretion and claudin-1 expression and preserving the distribution of occludin. Furthermore, we also observed that RGal accelerated the wound healing in Caco-2 epithelial cell line. In conclusion, RGal ameliorates intestinal barrier function in vivo and in vitro and may represent an attractive and promising molecule for the therapeutic management of ulcerative colitis.
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DOI：
10.1152/ajpgi.00491.2005
发表时间：
2006-08-01
影响因子：
4.5
作者：
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