ADSCs stimulated by resistin promote breast cancer cell malignancy via CXCL5 in a breast cancer coculture model.
ADSCs stimulated by resistin promote breast cancer cell malignancy via CXCL5 in a breast cancer coculture model.
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DOI：
10.1038/s41598-022-19290-6
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发表时间：
2022-09-14
影响因子：
4.6
通讯作者：
Yuan, Shyng-Shiou F.
中科院分区：
文献类型：
--
作者：
Wang, Yen-Yun;Hung, Amos C.;Wu, Yi-Chia;Lo, Steven;Chen, Huan-Da;Chen, Yuk-Kwan;Hsieh, Ya-Ching;Hu, Stephen Chu-Sung;Hou, Ming-Feng;Yuan, Shyng-Shiou F.
The tumor microenvironment represents one of the main obstacles in breast cancer treatment owing to the presence of heterogeneous stromal cells, such as adipose-derived stem cells (ADSCs), that may interact with breast cancer cells and promote cancer development. Resistin is an adipocytokine associated with adverse breast cancer progression; however, its underlying mechanisms in the context of the breast tumor microenvironment remain largely unidentified. Here, we utilized a transwell co-culture model containing patient-derived ADSCs and breast cancer cell lines to investigate their potential interaction, and observed that breast cancer cells co-cultured with resistin-treated ADSCs (R-ADSCs) showed enhanced cancer cell growth and metastatic ability. Screening by proteome arrays revealed that C-X-C motif chemokine ligand 5 (CXCL5) was released in the conditioned medium of the co-culture system, and phosphorylated ERK was increased in breast cancer cells after co-culture with R-ADSCs. Breast cancer cells treated with the recombinant proteins of CXCL5 showed similarly enhanced cell migration and invasion ability as occurred in the co-culture model, whereas application of neutralizing antibodies against CXCL5 reversed these phenomena. The orthotopic xenograft in mice by breast cancer cells after co-culture with R-ADSCs had a larger tumor growth and more CXCL5 expression than control. In addition, clinical analysis revealed a positive correlation between the expression of resistin and CXCL5 in both tumor tissues and serum specimens of breast cancer patients. The current study suggests that resistin-stimulated ADSCs may interact with breast cancer cells in the tumor microenvironment via CXCL5 secretion, leading to breast cancer cell malignancy.
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影响因子：
5.9
作者：
Balaban S;Shearer RF;Lee LS;van Geldermalsen M;Schreuder M;Shtein HC;Cairns R;Thomas KC;Fazakerley DJ;Grewal T;Holst J;Saunders DN;Hoy AJ
通讯作者：
Hoy AJ
影响因子：
7.5
作者：
Koellensperger E;Bonnert LC;Zoernig I;Marmé F;Sandmann S;Germann G;Gramley F;Leimer U
通讯作者：
Leimer U
DOI：
10.1186/s13058-016-0740-2
发表时间：
2016-08-11
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Bussard KM;Mutkus L;Stumpf K;Gomez-Manzano C;Marini FC
通讯作者：
Marini FC
影响因子：
3.8
作者：
Avtanski, Dimiter;Garcia, Anabel;Poretsky, Leonid
通讯作者：
Poretsky, Leonid
影响因子：
4.7
作者：
Lee, Yi-Chen;Chen, Yun-Ju;Yuan, Shyng-Shiou F.
通讯作者：
Yuan, Shyng-Shiou F.