Piezo1 plays a role in optic nerve head astrocyte reactivity.
Piezo1 plays a role in optic nerve head astrocyte reactivity.
复制标题
Piezo1在视神经乳头星形胶质细胞反应中起作用。
DOI：
10.1016/j.exer.2021.108445
复制
发表时间：
2021-03
影响因子：
3.4
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Liu J;Yang Y;Liu Y
Piezo1（也称为 Fam38A）是各种细胞类型中机械转导所需的机械传感通道。在星形胶质细胞中，Piezo1 激活与中枢神经系统神经变性的发病机制相关。已在小鼠视神经乳头星形胶质细胞中检测到 Piezo1 的表达，但 Piezo1 的功能作用尚未确定。在这项研究中，我们研究了 Piezo1 在视神经乳头星形胶质细胞反应性中的作用。培养原代小鼠视神经头星形胶质细胞并进行机械拉伸。通过定量PCR和免疫细胞化学染色测定Piezo1的表达水平。星形胶质细胞进一步用 Yoda1（一种特定的 Piezo1 激动剂）处理。在 Yoda1 处理的细胞中测定细胞内钙浓度以及 F-肌动蛋白和纤连蛋白的表达。我们发现机械拉伸激活视神经乳头星形胶质细胞中的 Piezo1。 Yoda1 以剂量依赖性方式诱导强烈的 Ca2+ 反应。此外，Yoda1 处理的细胞显示出 F-肌动蛋白细胞骨架的重新分布和纤连蛋白表达的增加，这表明星形胶质细胞具有反应性。我们的结果表明 Piezo1 对机械刺激做出反应并在星形胶质细胞反应中发挥作用。这项研究为视神经乳头星形胶质细胞机械转导提供了新的见解。
Piezo1 (also known as Fam38A) is a mechanosensing channel required for mechanotransduction in various cell types. In astrocytes, Piezo1 activation is associated with the pathogenesis of central nervous system neurodegeneration. Expression of Piezo1 has been detected in mouse optic nerve head astrocytes, however, the functional role of Piezo1 has not been identified. In this study, we investigated the role of Piezo1 in optic nerve head astrocyte reactivity. Primary mouse optic nerve head astrocytes were cultured and subject to mechanical stretch. The expression level of Piezo1 was determined by quantitative PCR and immunocytochemistry staining. Astrocytes were further treated with Yoda1, a specific Piezo1 agonist. The intracellular calcium concentration and expression of F-actin and fibronectin were determined in Yoda1 treated cells. We found that mechanical stretch activated Piezo1 in optic nerve head astrocytes. Yoda1 induced robust Ca2+ responses in a dose dependent manner. In addition, Yoda1 treated cells showed a redistribution of F-actin cytoskeleton and an increased expression of fibronectin which indicated astrocyte reactivity. Our results suggest that Piezo1 responses to mechanical stimulation and plays a role in astrocyte reactivity. This study provides new insights into optic nerve head astrocyte mechanotransduction.
登录
查看更多内容
TRPV1阳离子通道的激活有助于压力诱导的星形胶质细胞迁移。
影响因子：
6.2
作者：
Ho, Karen W.;Lambert, Wendi S.;Calkins, David J.
通讯作者：
Calkins, David J.
影响因子：
6.2
作者：
Beckel, Jonathan M.;Argall, Arthur J.;Lim, Jason C.;Xia, Jingsheng;Lu, Wennan;Coffey, Erin E.;Macarak, Edward J.;Shahidullah, Mohammed;Delamere, Nicholas A.;Zode, Gulab S.;Sheffield, Val C.;Shestopalov, Valery I.;Laties, Alan M.;Mitchell, Claire H.
通讯作者：
Mitchell, Claire H.
影响因子：
6.2
作者：
Sun, Daniel;Qu, Juan;Jakobs, Tatjana C.
通讯作者：
Jakobs, Tatjana C.
影响因子：
64.8
作者：
Coste, Bertrand;Xiao, Bailong;Santos, Jose S.;Syeda, Ruhma;Grandl, Joerg;Spencer, Kathryn S.;Kim, Sung Eun;Schmidt, Manuela;Mathur, Jayanti;Dubin, Adrienne E.;Montal, Mauricio;Patapoutian, Ardem
通讯作者：
Patapoutian, Ardem
影响因子：
2.9
作者：
Satoh, Kazuki;Hata, Mitsumi;Yamada, Tatsuo
通讯作者：
Yamada, Tatsuo