A method for assessing the microvasculature in a murine tumor model using contrast-enhanced ultrasonography.
A method for assessing the microvasculature in a murine tumor model using contrast-enhanced ultrasonography.
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一种使用对比增强的超声检查来评估鼠肿瘤模型中微脉管系统的方法。
DOI：
10.7863/jum.2008.27.12.1699
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发表时间：
2008-12
期刊：
影响因子：
--
通讯作者：
Yankeelov TE
中科院分区：
文献类型：
--
作者：
Loveless ME;Li X;Huamani J;Lyshchik A;Dawant B;Hallahan D;Gore JC;Yankeelov TE
本研究的目的是开发一种使用超声造影评估乳腺癌临床前模型中肿瘤血管分布的方法。在8只小鼠的后肢上注射67NR乳腺癌细胞，8天后用超声成像。给小鼠注射超声造影剂（UCA），并在高功率“破坏”脉冲之前和之后记录多个平行平面中每个平面的后向散射回波的图像序列。从这些，最大的对比度增强（在每个时间过程中）的数据图构建每个像素，这使得重建高分辨率共配准部分到一个三维（3D）体积反映肿瘤血管。进行了额外的研究，以确定图像增强的持续时间和可重复性，并将图像与传统的3D能量多普勒测量相关联。发现UCA在体内的寿命为4.3 ± 1.09分钟（平均值± SD）。3D对比增强超声技术产生的图像与特定区域的能量多普勒图像相关性良好，但也描绘了能量多普勒信号周围的其他血流区域。每只动物中央切片体素测量值之间的平均相关系数为0.64 ± 0.07（P <0.01）。此外，每只动物的连续研究具有重现性。在乳腺癌的临床前模型中产生肿瘤血管分布的高分辨率体积评估的方法显示与血流的其他超声测量相关，这可以提供对微血管的更高灵敏度。
The purpose of this study was to develop a method for assessing tumor vascularity in a preclinical model of breast cancer using contrast-enhanced ultrasonography. Eight mice were injected with 67NR breast cancer cells on their hind limbs and imaged with ultrasonography 8 days later. Mice were injected with an ultrasound contrast agent (UCA), and a sequence of images of the resultant backscattered echoes was recorded before and after high-power “destruction” pulses for each of multiple parallel planes. From these, data maps of the maximum contrast enhancement (within each time course) were constructed for each pixel, which enabled reconstruction of high-resolution coregistered sections into a 3-dimensional (3D) volume reflecting tumor vascularity. Additional studies were performed to determine the duration and repeatability of image enhancement, and images were correlated with conventional 3D power Doppler measurements. The lifetime of the UCA in vivo was found to be 4.3 ± 1.09 minutes (mean ± SD). The 3D contrast-enhanced ultrasonographic technique produced images that correlated well with power Doppler images in specific regions but also depicted additional regions of flow surrounding the power Doppler signal. The mean correlation coefficient between voxel measurements of the central slice for each animal was 0.64 ± 0.07 (P < .01). In addition, sequential studies in each animal were reproducible. A method producing high-resolution volumetric assessments of tumor vascularity in a preclinical model of breast cancer is shown that correlates with other ultrasonographic measures of blood flow, which may provide greater sensitivity to the microvasculature.
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影响因子：
2.6
作者：
Cosgrove, D
通讯作者：
Cosgrove, D
DOI：
10.1109/tuffc.2005.1397351
发表时间：
2005-01-01
影响因子：
3.6
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
9
作者：
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通讯作者：
FOLKMAN, J