Complete genome sequence and comparative analysis of the wild-type commensal Escherichia coli strain SE11 isolated from a healthy adult.
Complete genome sequence and comparative analysis of the wild-type commensal Escherichia coli strain SE11 isolated from a healthy adult.
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DOI：
10.1093/dnares/dsn026
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发表时间：
2008-12
期刊：
影响因子：
4.1
通讯作者：
Hattori, Masahira
中科院分区：
文献类型：
--
作者：
Oshima, Kenshiro;Toh, Hidehlro;Ogura, Yoshitoshi;Sasamoto, Hiroyuki;Morita, Hidetoshi;Park, Sang-Hee;Ooka, Tadasuke;Iyoda, Sunao;Taylor, Todd D.;Hayashi, Tetsuya;Itoh, Kikuji;Hattori, Masahira
我们对最近从健康成人粪便中分离到的一株共生大肠杆菌SE11(O152：H28)的基因组进行了测序和分析，并将其归入大肠杆菌系统发育群B1。SE11含有一条4.8Mb的染色体，编码4679个蛋白质编码基因和6个编码323个蛋白质编码基因的质粒。SE11基因没有一个与已知的编码在致病性大肠杆菌菌株中发现的噬菌体和质粒携带的毒力因子的基因序列相似。与实验室毒株K-12 MG1655的比较基因组分析发现，这两个非致病毒株之间有62个保守较差的基因，而MG1655中没有1186个基因。SE11中的这些基因大多编码在染色体或质粒上的大插入区，尤其是菌毛和自体转运蛋白基因，它们是细胞表面的附属物，对细菌与宿主细胞的黏附能力和细菌接合有很大贡献。这些数据表明，SE11可能已经进化为获得和积累了有利于肠道细胞稳定定植的功能，并且黏附相关功能对于大肠杆菌在人类肠道栖息地的共生具有重要意义。
We sequenced and analyzed the genome of a commensal Escherichia coli (E. coli) strain SE11 (O152:H28) recently isolated from feces of a healthy adult and classified into E. coli phylogenetic group B1. SE11 harbored a 4.8 Mb chromosome encoding 4679 protein-coding genes and six plasmids encoding 323 protein-coding genes. None of the SE11 genes had sequence similarity to known genes encoding phage- and plasmid-borne virulence factors found in pathogenic E. coli strains. The comparative genome analysis with the laboratory strain K-12 MG1655 identified 62 poorly conserved genes between these two non-pathogenic strains and 1186 genes absent in MG1655. These genes in SE11 were mostly encoded in large insertion regions on the chromosome or in the plasmids, and were notably abundant in genes of fimbriae and autotransporters, which are cell surface appendages that largely contribute to the adherence ability of bacteria to host cells and bacterial conjugation. These data suggest that SE11 may have evolved to acquire and accumulate the functions advantageous for stable colonization of intestinal cells, and that the adhesion-associated functions are important for the commensality of E. coli in human gut habitat.
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影响因子：
2.8
作者：
Chen, Q;Savarino, SJ;Venkatesan, MM
通讯作者：
Venkatesan, MM
影响因子：
5.1
作者：
Clermont, Olivier;Lescat, Mathilde;Denamur, Erick
通讯作者：
Denamur, Erick
影响因子：
3.1
作者：
Dudley, EG;Abe, C;Nataro, JP
通讯作者：
Nataro, JP
影响因子：
10.7
作者：
Escobar-Páramo, P;Clermont, O;Denamur, E
通讯作者：
Denamur, E
影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.