Protocol for in utero injection to investigate Zika virus-related fetal microcephaly in mice.
Protocol for in utero injection to investigate Zika virus-related fetal microcephaly in mice.
复制标题
研究小鼠寨卡病毒相关胎儿小头畸形的子宫内注射方案。
DOI：
10.1016/j.xpro.2021.101057
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发表时间：
2022-03-18
期刊：
影响因子：
--
通讯作者：
Zeng J
中科院分区：
文献类型：
--
作者：
Dong S;Xie X;Chen Y;Long G;Liang Q;Zhao Z;Zeng J
寨卡病毒与包括小头畸形在内的先天性缺陷有关。一个能够概括大多数小头症相关表型的感染模型对于理解寨卡病毒的发病机制至关重要。在这里，我们提出了一种方案，通过反向遗传系统从感染性克隆中产生寨卡病毒，随后用获救的寨卡病毒在怀孕小鼠中进行胚胎脑感染。我们优化了几个方面的程序，包括病毒抢救和子宫内注射。该方案促进了病毒诱导的皮质发育缺陷的可重复性研究。有关使用和执行本方案的完整详细信息，请参阅感染性克隆制备寨卡病毒（ZIKV）工作流程子宫内注射寨卡病毒方案注射寨卡病毒后脑部取样细节寨卡病毒相关小头畸形表型分析指南寨卡病毒（ZIKV）与包括小头畸形在内的先天性缺陷有关。一个能够概括大多数小头症相关表型的感染模型对于理解寨卡病毒的发病机制至关重要。在这里，我们提出了一种方案，通过反向遗传系统从感染性克隆中产生寨卡病毒，随后用获救的寨卡病毒在怀孕小鼠中进行胚胎脑感染。我们优化了几个方面的程序，包括病毒抢救和子宫内注射。该方案促进了病毒诱导的皮质发育缺陷的可重复性研究。
Zika virus (ZIKV) is linked to congenital defects including microcephaly. An infection model that can recapitulate most microcephaly-related phenotypes is crucial for understanding ZIKV pathogenesis. Here, we present a protocol to generate ZIKV from an infectious clone through a reverse genetic system and subsequently perform embryonic brain infection with the rescued ZIKV in pregnant mice. We optimized several aspects of the procedures including virus rescue and in utero injection. This protocol facilitates reproducible investigation of virus-induced cortical development defects. For complete details on the use and execution of this protocol, please refer to Workflow of Zika virus (ZIKV) preparation from an infectious clone Protocol for in utero ZIKV injection Details for brain sampling after ZIKV injection Guidelines for ZIKV-related microcephaly phenotype analysis Zika virus (ZIKV) is linked to congenital defects including microcephaly. An infection model that can recapitulate most microcephaly-related phenotypes is crucial for understanding ZIKV pathogenesis. Here, we present a protocol to generate ZIKV from an infectious clone through a reverse genetic system and subsequently perform embryonic brain infection with the rescued ZIKV in pregnant mice. We optimized several aspects of the procedures including virus rescue and in utero injection. This protocol facilitates reproducible investigation of virus-induced cortical development defects.
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E90 亚单位疫苗可保护小鼠免受寨卡病毒感染和小头畸形。
影响因子：
7.1
作者：
Zhu X;Li C;Afridi SK;Zu S;Xu JW;Quanquin N;Yang H;Cheng G;Xu Z
通讯作者：
Xu Z
DOI：
10.3791/236
发表时间：
2007-01-01
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Walantus, William;Elias, Laura;Kriegstein, Arnold
通讯作者：
Kriegstein, Arnold
影响因子：
4.6
作者：
Zhao F;Xu Y;Lavillette D;Zhong J;Zou G;Long G
通讯作者：
Long G
影响因子：
30.3
作者：
Shan C;Xie X;Muruato AE;Rossi SL;Roundy CM;Azar SR;Yang Y;Tesh RB;Bourne N;Barrett AD;Vasilakis N;Weaver SC;Shi PY
通讯作者：
Shi PY
影响因子：
1.3
作者：
Yamada, Makiko;Hatta, Toshihisa;Otani, Hiroki
通讯作者：
Otani, Hiroki