Proteomic Identification of an Upregulated Isoform of Annexin A3 in the Spinal Cords of Rats in a Neuropathic Pain Model.
Proteomic Identification of an Upregulated Isoform of Annexin A3 in the Spinal Cords of Rats in a Neuropathic Pain Model.
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大鼠神经病理性疼痛模型脊髓中膜联蛋白 A3 上调同种型的蛋白质组学鉴定
DOI：
10.3389/fnins.2017.00484
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发表时间：
2017
影响因子：
4.3
通讯作者：
Guo Q
中科院分区：
文献类型：
--
作者：
Zou W;Xu W;Song Z;Zhong T;Weng Y;Huang C;Li M;Zhang C;Zhan X;Guo Q
神经性疼痛（NP）是由神经损伤或周围或中枢神经系统的紊乱引起的。神经损伤引起外周和中枢神经系统中的致敏机制的激活，其诱导感觉神经中的转录和转录后改变。然而，NP的潜在机制仍然难以捉摸。在这项研究中，双向凝胶电泳（2DGE）为基础的比较蛋白质组学确定了38个差异凝胶点，和15个差异表达的蛋白质（DEPs）之间的假手术和慢性压迫性损伤（CCI）诱导的神经病理性疼痛大鼠。免疫印迹和免疫荧光染色显示，CCI后Annexin A3（ANXA 3）表达明显增加。鞘内给予递送ANXA 3 shRNA的慢病毒（LV-shANXA 3）以确定ANXA 3对大鼠中CCI诱导的神经病理性疼痛模型中的异常性疼痛和痛觉过敏的镇痛作用。进一步的研究表明，LV-shANXA 3逆转ANXA 3的上调，减轻CCI诱导的机械异常性疼痛和热痛觉过敏。研究表明ANXA 3可能在神经病理性疼痛中发挥重要作用。
Neuropathic pain (NP) is induced by nerve damage or a disturbance in the peripheral or central nervous systems. Nerve damage causes the activation of sensitizing mechanisms in the peripheral and central nervous systems, which induces transcriptional and post-transcriptional alterations in sensory nerves. However, the underlying mechanisms of NP remain elusive. In the study, Two-dimensional gel electrophoresis (2DGE)-based comparative proteomics identified 38 differential gel spots, and 15 differentially expressed proteins (DEPs) between the sham and the chronic constriction injury (CCI)-induced neuropathic pain rats. Of them, Annexin A3 (ANXA3) was significantly increased after CCI with Western blot analysis and immunofluorescence imaging. A lentivirus delivering ANXA3 shRNA (LV-shANXA3) was administered intrathecally to determine the analgesic effects of ANXA3 on allodynia and hyperalgesia in a CCI-induced neuropathic pain model in rats. Further study showed that LV-shANXA3 reversed the upregulation of ANXA3, alleviated CCI-induced mechanical allodynia and thermal hyperalgesia. The study indicated that ANXA3 may play an important role in neuropathic pain.
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周围神经损伤后脊髓背角突触体相关蛋白的蛋白质组。
影响因子：
3.4
作者：
Singh, Om V.;Yaster, Myron;Xu, Ji-Tian;Guan, Yun;Guan, Xiaowei;Dharmarajan, Arun M.;Raja, Srinivasa N.;Zeitlin, Pamela L.;Tao, Yuan-Xiang
通讯作者：
Tao, Yuan-Xiang
神经损伤引起的脊髓神经元中乳胶素表达的丧失有助于神经性疼痛的发展。
影响因子：
3.7
作者：
Kühlein HN;Tegeder I;Möser C;Lim HY;Häussler A;Spieth K;Jennes I;Marschalek R;Beckhaus T;Karas M;Fauth M;Ehnert C;Geisslinger G;Niederberger E
通讯作者：
Niederberger E
影响因子：
5.3
作者：
Avenali, Luca;Narayanan, Pratibha;Schmidt, Manuela
通讯作者：
Schmidt, Manuela
影响因子：
7
作者：
Rouwette, Tom;Sondermann, Julia;Schmidt, Manuela
通讯作者：
Schmidt, Manuela
影响因子：
9.3
作者：
Schäfer S;Berger JV;Deumens R;Goursaud S;Hanisch UK;Hermans E
通讯作者：
Hermans E