Proteome of synaptosome-associated proteins in spinal cord dorsal horn after peripheral nerve injury.
Proteome of synaptosome-associated proteins in spinal cord dorsal horn after peripheral nerve injury.
复制标题
周围神经损伤后脊髓背角突触体相关蛋白的蛋白质组。
DOI：
10.1002/pmic.200800636
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发表时间：
2009-03
期刊：
影响因子：
3.4
通讯作者：
Tao, Yuan-Xiang
中科院分区：
文献类型：
--
作者：
Singh, Om V.;Yaster, Myron;Xu, Ji-Tian;Guan, Yun;Guan, Xiaowei;Dharmarajan, Arun M.;Raja, Srinivasa N.;Zeitlin, Pamela L.;Tao, Yuan-Xiang
关键词：
周围神经损伤可能导致脊髓细胞信号的神经适应性变化，这些变化被认为有助于神经病理性疼痛的中枢机制。本研究采用双向电泳（2-DE）蛋白质组学技术检测单侧第五脊神经损伤（SNI）后脊髓背角突触体相关蛋白表达的变化。在手术后第14天收获SNI或假手术同侧的第五腰椎背角，并分离总可溶性和突触体部分。通过2-DE解析来自突触体组分的蛋白质。我们确定了27个蛋白质，显示不同的表达水平后SNI，包括蛋白质参与传递和调节有害信息，细胞代谢，膜受体运输，氧化应激，细胞凋亡和变性。随机选择27种蛋白质中的6种，并通过Western印迹分析在突触体组分中进一步验证。出乎意料的是，Western印迹分析显示，在SNI后，总可溶性级分中仅一种蛋白质表现出显著的表达变化。这些数据表明，周围神经损伤不仅改变蛋白质表达，而且改变蛋白质在背角细胞中的亚细胞分布。这些变化可能参与维持神经病理性疼痛的中枢机制。
Peripheral nerve injury may lead to neuroadaptive changes of cellular signals in spinal cord that are thought to contribute to central mechanisms underlying neuropathic pain. Here we used a two-dimensional electrophoresis (2-DE)-based proteomic technique to determine the global expression changes of synaptosome-associated proteins in spinal cord dorsal horn after unilateral fifth spinal nerve injury (SNI). The fifth lumbar dorsal horns ipsilateral to SNI or sham surgery were harvested on day 14 post-surgery, and the total soluble and synaptosomal fractions were isolated. The proteins derived from the synaptosomal fraction were resolved by 2-DE. We identified 27 proteins that displayed different expression levels after SNI, including proteins involved in transmission and modulation of noxious information, cellular metabolism, membrane receptor trafficking, oxidative stress, apoptosis, and degeneration. Six of the 27 proteins were chosen randomly and further validated in the synaptosomal fraction by Western blot analysis. Unexpectedly, Western blot analysis showed that only one protein in the total soluble fraction exhibited a significant expression change after SNI. The data indicate that peripheral nerve injury changes not only protein expression but also protein subcellular distribution in dorsal horn cells. These changes might participate in the central mechanism that underlies the maintenance of neuropathic pain.
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影响因子：
7.2
作者：
Drake, JM;Friis, RR;Dharmarajan, AM
通讯作者：
Dharmarajan, AM
影响因子：
8.8
作者：
Chen, SR;Khan, GM;Pan, HL
通讯作者：
Pan, HL
影响因子：
2.7
作者：
Lee, SC;Yoon, TG;Kim, HJ
通讯作者：
Kim, HJ
DOI：
10.1152/ajpendo.00053.2007
发表时间：
2007-07-01
影响因子：
5.1
作者：
Dominy, John E., Jr.;Hwang, Jesse;Stipanuk, Martha H.
通讯作者：
Stipanuk, Martha H.
影响因子：
3.3
作者：
Guan Y;Yaster M;Raja SN;Tao YX
通讯作者：
Tao YX