Molecular basis of an agarose metabolic pathway acquired by a human intestinal symbiont.
Molecular basis of an agarose metabolic pathway acquired by a human intestinal symbiont.
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DOI：
10.1038/s41467-018-03366-x
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发表时间：
2018-03-13
影响因子：
16.6
通讯作者：
Abbott DW
中科院分区：
文献类型：
--
作者：
Pluvinage B;Grondin JM;Amundsen C;Klassen L;Moote PE;Xiao Y;Thomas D;Pudlo NA;Anele A;Martens EC;Inglis GD;Uwiera RER;Boraston AB;Abbott DW
在红藻中，最丰富的主要细胞壁多糖是混合半乳聚糖琼脂，其中琼脂糖是一种常见的成分。虽然琼脂糖的生物转化主要由生活在海洋中的细菌催化，但琼脂酶已在栖息于各种陆地生态系统（包括人类肠道）的微生物中发现。在这里，我们全面定义了人体肠道细菌单形拟杆菌（Bu）NP 1的琼脂分解途径的结构-功能关系。使用重组琼脂酶从Bu NP 1完全去琼脂糖，我们证明了非琼脂分解Bu菌株可以生长在从琼脂糖释放的GAL上。这种关系强调了肠道细菌对稀有营养物质的利用是通过获得高度特异性的酶来促进的，这些酶释放了包含在不寻常的多糖中的难以获得的碳水化合物资源。有趣的是，琼脂糖分解途径在地理上不同的人类微生物组中分布不同，反映了人类消耗琼脂糖的复杂历史背景。多糖是海藻的主要结构细胞壁和能量储存分子。在这里，作者展示了人类肠道细菌单形拟杆菌如何选择性地利用地理限制的膳食多糖琼脂糖，从而深入了解碳水化合物代谢如何在人类微生物组中演变。
In red algae, the most abundant principal cell wall polysaccharides are mixed galactan agars, of which agarose is a common component. While bioconversion of agarose is predominantly catalyzed by bacteria that live in the oceans, agarases have been discovered in microorganisms that inhabit diverse terrestrial ecosystems, including human intestines. Here we comprehensively define the structure–function relationship of the agarolytic pathway from the human intestinal bacterium Bacteroides uniformis (Bu) NP1. Using recombinant agarases from Bu NP1 to completely depolymerize agarose, we demonstrate that a non-agarolytic Bu strain can grow on GAL released from agarose. This relationship underscores that rare nutrient utilization by intestinal bacteria is facilitated by the acquisition of highly specific enzymes that unlock inaccessible carbohydrate resources contained within unusual polysaccharides. Intriguingly, the agarolytic pathway is differentially distributed throughout geographically distinct human microbiomes, reflecting a complex historical context for agarose consumption by human beings. Polysaccharides are the primary structural cell wall and energy storage molecules of seaweed. Here, the authors show how the geographically restricted dietary polysaccharide agarose is selectively utilized by the human intestinal bacterium Bacteroides uniformis, providing insight into how carbohydrate metabolism evolves within the human microbiome.
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影响因子：
5.4
作者：
Fu XT;Kim SM
通讯作者：
Kim SM
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
DOI：
10.1007/s00018-016-2242-x
发表时间：
2016-07
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Foley MH;Cockburn DW;Koropatkin NM
通讯作者：
Koropatkin NM
影响因子：
14.9
作者：
Davis IW;Leaver-Fay A;Chen VB;Block JN;Kapral GJ;Wang X;Murray LW;Arendall WB 3rd;Snoeyink J;Richardson JS;Richardson DC
通讯作者：
Richardson DC
影响因子：
3.4
作者：
Baos, S. C.;Phillips, D. B.;Berry, M.
通讯作者：
Berry, M.