Adipocyte DIO2 Expression Increases in Human Obesity but Is Not Related to Systemic Insulin Sensitivity.
Adipocyte DIO2 Expression Increases in Human Obesity but Is Not Related to Systemic Insulin Sensitivity.
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DOI：
10.1155/2018/2464652
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发表时间：
2018
影响因子：
4.3
通讯作者：
Hsueh W
中科院分区：
文献类型：
--
作者：
Bradley D;Liu J;Blaszczak A;Wright V;Jalilvand A;Needleman B;Noria S;Renton D;Hsueh W
由DIO 2编码的II型脱碘酶（D2）催化T4转化为生物活性T3。T3不仅刺激适应性产热，而且影响脂肪组织（AT）脂质积累、线粒体功能、炎症和潜在的全身代谢。尽管在棕色AT中更好地定义，但DIO 2表达在白色AT中的确切作用在很大程度上仍然未知，数据仅来自全脂肪。因此，本研究的目的是确定DIO 2的皮下（SAT）和内脏（VAT）脂肪细胞特异性基因表达是否在肥胖和瘦型患者之间存在差异，以及这些差异是否与线粒体功能、脂肪酸通量、炎性细胞因子/脂肪因子以及最终胰岛素敏感性的改变有关。因此，分离73名肥胖受试者和21名瘦受试者的脂肪细胞并进行基因表达分析。我们的研究结果表明，肥胖的人相比，瘦的人增加了脂肪细胞特异性的DIO 2在SAT和VAT的表达。虽然较高的DIO 2与脂肪酸合成/氧化和线粒体功能降低密切相关，但我们发现与促炎细胞因子或胰岛素抵抗无关，并且基于糖尿病状态没有差异。我们的研究结果表明，脂肪细胞衍生的DIO 2可能在体重维持中发挥作用，但可能不是肥胖相关胰岛素抵抗的主要贡献者。
Deiodinase type II (D2), encoded by DIO2, catalyzes the conversion of T4 to bioactive T3. T3 not only stimulates adaptive thermogenesis but also affects adipose tissue (AT) lipid accumulation, mitochondrial function, inflammation, and potentially systemic metabolism. Although better defined in brown AT, the precise role of DIO2 expression in white AT remains largely unknown, with data derived only from whole fat. Therefore, the purpose of this study was to determine whether subcutaneous (SAT) and visceral (VAT) adipocyte-specific gene expression of DIO2 differs between obese and lean patients and whether these differences relate to alterations in mitochondrial function, fatty acid flux, inflammatory cytokines/adipokines, and ultimately insulin sensitivity. Accordingly, adipocytes of 73 obese and 21 lean subjects were isolated and subjected to gene expression analyses. Our results demonstrate that obese compared to lean human individuals have increased adipocyte-specific DIO2 expression in both SAT and VAT. Although higher DIO2 was strongly related to reduced fatty acid synthesis/oxidation and mitochondrial function, we found no relationship to proinflammatory cytokines or insulin resistance and no difference based on diabetic status. Our results suggest that adipocyte-derived DIO2 may play a role in weight maintenance but is likely not a major contributor to obesity-related insulin resistance.
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DOI：
10.1016/j.biocel.2011.05.016
发表时间：
2011-10
影响因子：
4
作者：
Arrojo e Drigo, Rafael;Bianco, Antonio C.
通讯作者：
Bianco, Antonio C.
DOI：
10.1001/jama.2014.732
发表时间：
2014-02-26
期刊：
JAMA
影响因子：
--
作者：
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通讯作者：
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影响因子：
4.4
作者：
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通讯作者：
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影响因子：
15.1
作者：
Kelley, Keith W.;Weigent, Douglas A.;Kooijman, Ron
通讯作者：
Kooijman, Ron
影响因子：
64.8
作者：
Houstis, N;Rosen, ED;Lander, ES
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Lander, ES