Characterization by Suppression Subtractive Hybridization of Transcripts That Are Differentially Expressed in Leaves of Anthracnose-Resistant Ramie Cultivar.
Characterization by Suppression Subtractive Hybridization of Transcripts That Are Differentially Expressed in Leaves of Anthracnose-Resistant Ramie Cultivar.
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DOI：
10.1007/s11105-011-0361-y
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发表时间：
2012
影响因子：
2.1
通讯作者：
Peng Dingxiang
中科院分区：
文献类型：
--
作者：
Wang Xuxia;Chen Jie;Wang Bo;Liu Lijun;Jiang Hui;Tang Diluo;Peng Dingxiang
关键词：
为筛选苎麻（Boehmeria nivea L. Gaud）抗炭疽病相关基因，以抗炭疽病品种花竹号为材料，采用抑制减法杂交技术构建cDNA文库。4. 华珠号的dna。以感染炭疽菌的4株玉米为检测材料，对未感染的花竹号玉米的dna进行检测。4 .作为司机。测序分析和同源性检索表明，这些克隆包含132个单基因，根据拟南芥的分类划分为功能类别，其中包括14个假定的细胞功能。这132个基因包括35个抗病和抗逆性相关基因，包括热休克蛋白90、金属硫蛋白、PR-1.2蛋白、过氧化氢酶基因、WRKY家族基因和蛋白酶抑制剂样蛋白。通过反转录PCR和RNA凝胶印迹分析部分疾病相关基因。这些表达的序列标签是首次在苎麻中报道的与炭疽病抗性相关的表达序列标签。
For the purpose of screening putative anthracnose resistance-related genes of ramie (Boehmeria nivea L. Gaud), a cDNA library was constructed by suppression subtractive hybridization using anthracnose-resistant cultivar Huazhu no. 4. The cDNAs from Huazhu no. 4, which were infected with Colletotrichum gloeosporioides, were used as the tester and cDNAs from uninfected Huazhu no. 4 as the driver. Sequencing analysis and homology searching showed that these clones represented 132 single genes, which were assigned to functional categories, including 14 putative cellular functions, according to categories established for Arabidopsis. These 132 genes included 35 disease resistance and stress tolerance-related genes including putative heat-shock protein 90, metallothionein, PR-1.2 protein, catalase gene, WRKY family genes, and proteinase inhibitor-like protein. Partial disease-related genes were further analyzed by reverse transcription PCR and RNA gel blot. These expressed sequence tags are the first anthracnose resistance-related expressed sequence tags reported in ramie.
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