Histaminergic regulation of seasonal metabolic rhythms in Siberian hamsters.
Histaminergic regulation of seasonal metabolic rhythms in Siberian hamsters.
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DOI：
10.1016/j.physbeh.2011.02.035
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发表时间：
2011-06-01
影响因子：
2.9
通讯作者：
Ebling FJ
中科院分区：
文献类型：
--
作者：
I'anson H;Jethwa PH;Warner A;Ebling FJ
我们研究了是否组胺能音调有助于西伯利亚仓鼠的季节性分解代谢状态，通过确定消融组胺能神经元对食物摄入量，代谢率和体重的影响。由于大多数组胺能神经元表达食欲素受体，因此将与食欲素-B结合的核糖体毒素（皂草素）注入下丘脑腹侧结节乳头区。这不仅导致后下丘脑中组胺能神经元的75-80%损失，而且还导致其他食欲素受体表达细胞例如MCH神经元的一些损失。在长日合成代谢状态下，病变导致术后体重一过性下降，但仓鼠恢复并保持恒定体重，而假病变仓鼠的体重逐渐增加。与假手术组相比，受损仓鼠在黑暗期的VO 2显著更高，自发活动也倾向于更高。在短日的第二项研究中，假处理仓鼠显示出预期的季节性体重下降，但与长日研究一样，受损仓鼠的体重保持不变。病变仓鼠消耗更多的黑暗早期阶段和较少的光期间，由于在黑暗期间的用餐频率增加和减少餐量在光期间，他们的累计食物摄入量在他们的家笼是大于对照仓鼠。总之，消融下丘脑后部的食欲素反应细胞可通过防止季节性摄食减少来阻断短日照诱导的体重下降，这一证据与中枢组胺能机制有助于长期调节体重的假设一致。皂甙-食欲素-B毒素可消融约80%的结节乳头状体组胺能神经元。病变破坏了食欲和能量代谢的季节性节律。中枢组胺能机制有助于长期调节体重。
We investigated whether histaminergic tone contributes to the seasonal catabolic state in Siberian hamsters by determining the effect of ablation of histaminergic neurons on food intake, metabolic rate and body weight. A ribosomal toxin (saporin) conjugated to orexin-B was infused into the ventral tuberomammillary region of the hypothalamus, since most histaminergic neurons express orexin receptors. This caused not only 75–80% loss of histaminergic neurons in the posterior hypothalamus, but also some loss of other orexin-receptor expressing cells e.g. MCH neurons. In the long-day anabolic state, lesions produced a transient post-surgical decrease in body weight, but the hamsters recovered and maintained constant body weight, whereas weight gradually increased in sham-lesioned hamsters. VO2 in the dark phase was significantly higher in the lesioned hamsters compared to shams, and locomotor activity also tended to be higher. In a second study in short days, sham-treated hamsters showed the expected seasonal decrease in body weight, but weight remained constant in the lesioned hamsters, as in the long-day study. Lesioned hamsters consumed more during the early dark phase and less during the light phase due to an increase in the frequency of meals during the dark and decreased meal size during the light, and their cumulative food intake in their home cages was greater than in the control hamsters. In summary, ablation of orexin-responsive cells in the posterior hypothalamus blocks the short-day induced decline in body weight by preventing seasonal hypophagia, evidence consistent with the hypothesis that central histaminergic mechanisms contribute to long-term regulation of body weight. ► Saporin-orexin-B toxin ablated ~ 80% of tuberomammillary histaminergic neurons. ► Lesions disrupted seasonal rhythms of appetite and energy metabolism. ► Central histaminergic mechanisms contribute to long-term regulation of body weight.
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影响因子：
3.3
作者：
Gerashchenko, D;Blanco-Centurion, C;Shiromani, PJ
通讯作者：
Shiromani, PJ
影响因子：
3.2
作者：
Herwig, A.;Ivanova, E. A.;Loudon, A. S.
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Loudon, A. S.
影响因子：
2.5
作者：
ERICSON, H;WATANABE, T;KOHLER, C
通讯作者：
KOHLER, C
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4.8
作者：
Barrett, P.;van den Top, M.;Spanswick, D.
通讯作者：
Spanswick, D.
影响因子：
5
作者：
Hancock, AA;Bennani, YL;Yao, BB
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Yao, BB