Genome and evolution of the shade-requiring medicinal herb Panax ginseng.
Genome and evolution of the shade-requiring medicinal herb Panax ginseng.
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DOI：
10.1111/pbi.12926
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发表时间：
2018-11
影响因子：
13.8
通讯作者：
Yang TJ
中科院分区：
文献类型：
--
作者：
Kim NH;Jayakodi M;Lee SC;Choi BS;Jang W;Lee J;Kim HH;Waminal NE;Lakshmanan M;van Nguyen B;Lee YS;Park HS;Koo HJ;Park JY;Perumal S;Joh HJ;Lee H;Kim J;Kim IS;Kim K;Koduru L;Kang KB;Sung SH;Yu Y;Park DS;Choi D;Seo E;Kim S;Kim YC;Hyun DY;Park YI;Kim C;Lee TH;Kim HU;Soh MS;Lee Y;In JG;Kim HS;Kim YM;Yang DC;Wing RA;Lee DY;Paterson AH;Yang TJ
被誉为药材之王的人参，由于其生长缓慢、世代时间长、种子产量低、基因组结构复杂等缺点，阻碍了对其的研究。在这里，我们通过从头组装揭示了四倍体人参的基因组结构，代表2.98 Gbp，有59 352个注释基因。重测序数据表明，二倍体Panax物种在南亚与全球变暖有关，两个北美物种通过两次洲际迁徙进化而来。在与Apiaceae分化后，五加科（包括人参）发生了两次全基因组复制（WGD），其中较近的一次使人参具有越冬能力，使其能够在北半球广泛传播。功能和进化分析表明，具有重要药理意义的达玛烷型人参皂苷起源于人参，主要在茎组织中产生并运输到根；新进化的人参脂肪酸去饱和酶增加了耐冻性；叶绿素a/b结合蛋白基因前所未有的保留使得在弱光下进行有效的光合作用。基因组规模的代谢网络提供了人参皂苷生物合成的整体视图。本研究为通过基因组辅助育种或代谢工程提高人参药用价值提供了宝贵的资源。
Panax ginseng C. A. Meyer, reputed as the king of medicinal herbs, has slow growth, long generation time, low seed production and complicated genome structure that hamper its study. Here, we unveil the genomic architecture of tetraploid P. ginseng by de novo genome assembly, representing 2.98 Gbp with 59 352 annotated genes. Resequencing data indicated that diploid Panax species diverged in association with global warming in Southern Asia, and two North American species evolved via two intercontinental migrations. Two whole genome duplications (WGD) occurred in the family Araliaceae (including Panax) after divergence with the Apiaceae, the more recent one contributing to the ability of P. ginseng to overwinter, enabling it to spread broadly through the Northern Hemisphere. Functional and evolutionary analyses suggest that production of pharmacologically important dammarane‐type ginsenosides originated in Panax and are produced largely in shoot tissues and transported to roots; that newly evolved P. ginseng fatty acid desaturases increase freezing tolerance; and that unprecedented retention of chlorophyll a/b binding protein genes enables efficient photosynthesis under low light. A genome‐scale metabolic network provides a holistic view of Panax ginsenoside biosynthesis. This study provides valuable resources for improving medicinal values of ginseng either through genomics‐assisted breeding or metabolic engineering.
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影响因子：
14.9
作者：
Dai X;Zhao PX
通讯作者：
Zhao PX
影响因子：
5.3
作者：
Jayakodi M;Choi BS;Lee SC;Kim NH;Park JY;Jang W;Lakshmanan M;Mohan SVG;Lee DY;Yang TJ
通讯作者：
Yang TJ
Plant-mPLoc：增强植物蛋白亚细胞定位预测能力的自上而下策略
影响因子：
3.7
作者：
Chou KC;Shen HB
通讯作者：
Shen HB
影响因子：
12.3
作者：
Haas, Brian J.;Salzberg, Steven L.;Zhu, Wei;Pertea, Mihaela;Allen, Jonathan E.;Orvis, Joshua;White, Owen;Buell, C. Robin;Wortman, Jennifer R.
通讯作者：
Wortman, Jennifer R.
影响因子：
2
作者：
Choi, Hong-Il;Kim, Nam-Hoon;Yang, Tae-Jin
通讯作者：
Yang, Tae-Jin