Uremia reduces serum insulin-like growth factor I, increases insulin-like growth factor II, and modifies their serum protein binding.
Uremia reduces serum insulin-like growth factor I, increases insulin-like growth factor II, and modifies their serum protein binding.
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尿毒症会降低血清胰岛素样生长因子 I，增加胰岛素样生长因子 II，并改变其血清蛋白结合。
DOI：
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发表时间：
1982
影响因子：
5.8
通讯作者：
W. Daughaday
中科院分区：
文献类型：
--
作者：
A. Goldberg;B. Trivedi;J. Delmez;H. Harter;W. Daughaday
由于尿毒症与儿童生长发育障碍有关，已通过生物测定、放射受体分析(RRA)和放射免疫分析(RIA)对患者的血清生长原子素水平进行了研究。虽然生物检测表明低水平的生长原子素，但天然血清中的RRA和RIA水平却有所上升。我们通过胰岛素样生长因子I(IGF-I)RIA和胰岛素样生长因子II(IGF-II)特异性的大鼠胎盘膜RRA重新研究了这个话题。血清用酸性乙醇提取，回收生长抑素，排除生长抑素结合蛋白(SBP)。结果以与正常人类参考血清相比的相对效价表示。22例成人慢性血液透析患者血清IGF-I RIA测定结果为0.41°0.05U/ml(平均SE°SE)。正常成人血清的最低检测限值为0.58和1.84U/ml，而IGF-II RRA的检测结果显著升高(3.9°0.3U/ml)。正常成人血清的下限为0.39和1.05U/ml。为了测定有效的血清结合蛋白，我们分别孵育了2，10，25和100/xL.
Because uremia is associated with growth failure in children, serum levels of somatomedins have been studied in patients by bioassay, radioreceptor assay (RRA), and RIA. While bioassays have indicated low levels of somatomedin, RRA and RIA of native serum have been elevated. We reinvestigated this topic by insulin-like growth factor I (IGF-I) RIA and insulin-like growth factor II (IGF-II) specific rat placental membrane RRA. Sera were extracted with acid-ethanol, which recovers somatomedins and excludes somatomedin-binding proteins (SBP). Results are expressed as the relative potency compared to a normal human reference serum. The result of a serum IGF-I RIA of 22 adult patients undergoingchronic hemodialysis was 0.41 ° 0.05 U/ml (mean ° SE). Limits of normal adult sera are 0.58 and 1.84 U/ml. In contrast, the result of the IGF-II RRA was markedly elevated (3.9 ° 0.3 U/ml). Limits of normal adult serum are 0.39and 1.05 U/ml. To determine available serum binding protein, we incubated 2, 10, 25, and 100 /xl...
脐带血浆中生长调节素肽分布和生长调节素结合蛋白含量：与正常和垂体垂体血浆的比较。
DOI：
10.1210/jcem-54-2-223
发表时间：
1982
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Borsi,L;Rosenfeld,RG;Liu,F;Hintz,RL
通讯作者：
Hintz,RL
DOI：
10.1016/s0021-9258(18)34631-3
发表时间：
1982-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Massagué;M. Czech
通讯作者：
J. Massagué;M. Czech