Mutagenicity and alkylating activity of the aqueous chlorination products of humic acid and their molecular weight fractions.
Mutagenicity and alkylating activity of the aqueous chlorination products of humic acid and their molecular weight fractions.
复制标题
腐殖酸及其分子量级分的水性氯化产物的致突变性和烷基化活性。
DOI：
10.1016/0048-9697(89)90054-5
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Neton,J
中科院分区：
文献类型：
--
作者：
Agarwal,SC;Neton,J
在这些研究中用作模型底物的 Fluka 腐植酸对元素和含氧官能团进行了分析。它以 1:1 和 1:0.3 的 C:Cl 摩尔比进行氯化，随后通过超滤分离成分子量级分。使用 4-(对硝基苯甲基)吡啶分析冷冻干燥的氯化腐植酸和各自的分子量级分的 TOC、TOX、烷基化活性以及通过 Ames/沙门氏菌/微粒体测定对菌株 TA-98 和 TA-100 进行的致突变性。结果表明，在腐殖酸氯化过程中主要形成非挥发性、直接作用的诱变剂和/或烷化剂，并且这些试剂在不同分子量级分之间分布不均匀。诱变剂和烷基化剂的形成高度依赖于氯化水平和总有机碳。随着氯浓度在每摩尔碳 0.4-1.2 氯当量范围内增加，会产生更高水平的诱变和烷基化活性。然而，在含有非挥发物的冷冻干燥氯化腐植酸溶液或新鲜溶液中，这些活性随着pH值和储存时间的增加而逐渐降低，这显然是由于一些组分的降解和水解。
Fluka humic acid used as a model substrate in these studies was analysed for elemental and the oxygen-containing functional groups. It was chlorinated at C:Cl molar ratios of 1:1 and 1:0.3 and subsequently separated into molecular weight fractions by ultrafiltration. The freeze-dried, chlorinated humic acid and the respective molecular weight fractions were analyzed for TOC, TOX, alkylating activity using 4-(p-nitrobenzyl)pyridine and mutagenicity by the Ames/Salmonella/ microsome assay with strains TA-98 and TA-100. Results indicated that predominantly non-volatile, direct-acting mutagenic and/or alkylating agents were formed during humic acid chlorination and that these agents were unevenly distributed among the various molecular weight fractions. Formation of mutagenic and akylating agents were highly dependent upon level of chlorination and total organic carbon. Higher levels of mutagenic and alkylating activities were produced with increasing concentration of chlorine in the range of 0.4–1.2 chlorine equivalents per mole of carbon. However, both these activities in the freeze-dried, chlorinated humic acid solutions containing the non-volatiles or the fresh solutions decreased gradually with increasing pH and storage time, apparently due to degradation and hydrolysis of some of the components.
登录
查看更多内容
腐植酸氯化过程中诱变剂的形成。
影响因子：
11.4
作者：
K. Kringstad;P. Ljungquist;F. De Sousa;L. M. Stroemberg
通讯作者：
L. M. Stroemberg
影响因子：
11.4
作者：
MILLER, JW;UDEN, PC
通讯作者：
UDEN, PC
影响因子：
7.4
作者：
EPSTEIN, J;ROSENTHAL, RW;ESS, RJ
通讯作者：
ESS, RJ
影响因子：
12.7
作者：
T. Derouen;J. E. Diem
通讯作者：
J. E. Diem
影响因子：
2.7
作者：
AGARWAL, SC;VANDUUREN, BL;KNEIP, TJ
通讯作者：
KNEIP, TJ