Imaging neural activity in the ventral nerve cord of behaving adult Drosophila.
Imaging neural activity in the ventral nerve cord of behaving adult Drosophila.
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DOI：
10.1038/s41467-018-06857-z
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发表时间：
2018-10-22
影响因子：
16.6
通讯作者：
Ramdya P
中科院分区：
文献类型：
--
作者：
Chen CL;Hermans L;Viswanathan MC;Fortun D;Aymanns F;Unser M;Cammarato A;Dickinson MH;Ramdya P
为了了解控制肢体的神经回路，必须测量它们在行为过程中的活动。到目前为止，这一目标一直具有挑战性，因为肢体运动前区和运动回路在很大程度上无法在完整的移动动物中进行大规模记录这一限制对于脊椎动物和无脊椎动物模型都是如此。在这里，我们介绍了一种方法，从腹神经索（VNC）的行为成年果蝇的双光子功能成像。我们使用这种方法来揭示在梳理和行走过程中神经索群体的活动模式，并揭示moonwalker上行神经元（MAN），moonwalker下行神经元（MDN）和以前未表征的一类运动相关的A1下行神经元的功能编码。最后，我们开发了一种基因试剂来破坏间接飞行肌肉并促进对VNC的实验访问。总之，这些方法使直接调查与复杂的肢体运动的电路。果蝇腹侧神经索（VNC）的功能相当于脊椎动物的脊髓。本研究报告了一种双光子成像方法，用于记录行走和梳理成年苍蝇的VNC中的神经活动。
To understand neural circuits that control limbs, one must measure their activity during behavior. Until now this goal has been challenging, because limb premotor and motor circuits have been largely inaccessible for large-scale recordings in intact, moving animals—a constraint that is true for both vertebrate and invertebrate models. Here, we introduce a method for 2-photon functional imaging from the ventral nerve cord (VNC) of behaving adult Drosophila melanogaster. We use this method to reveal patterns of activity across nerve cord populations during grooming and walking and to uncover the functional encoding of moonwalker ascending neurons (MANs), moonwalker descending neurons (MDNs), and a previously uncharacterized class of locomotion-associated A1 descending neurons. Finally, we develop a genetic reagent to destroy the indirect flight muscles and to facilitate experimental access to the VNC. Taken together, these approaches enable the direct investigation of circuits associated with complex limb movements. The Drosophila ventral nerve cord (VNC) is functionally equivalent to the vertebrate spinal cord. This study reports a 2-photon imaging approach for recording neural activity in the VNC of walking and grooming adult flies.
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