Structural insights into ubiquinone biosynthesis in membranes.
Structural insights into ubiquinone biosynthesis in membranes.
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DOI：
10.1126/science.1246774
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发表时间：
2014-02-21
期刊：
影响因子：
--
通讯作者：
Li W
中科院分区：
文献类型：
--
作者：
Cheng W;Li W
Biosynthesis of ubiquinones requires the intramembrane UbiA enzyme, an archetypal member of a superfamily of prenyltransferases that generates lipophilic aromatic compounds. Mutations in eukaryotic superfamily members have been linked to cardiovascular degeneration and Parkinson's disease. To understand how quinones are produced within membranes, we report the crystal structures of an archaeal UbiA in its apo and substrate-bound states at 3.3 and 3.6Å resolution, respectively. The structures reveal nine transmembrane helices and an extra-membrane cap domain that surround a large central cavity containing the active site. To facilitate the catalysis inside membranes, UbiA has an unusual active site that opens laterally to the lipid bilayer. Our studies illuminate general mechanisms for substrate recognition and catalysis in the UbiA superfamily and rationalize disease-related mutations in humans.
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影响因子：
64.8
作者：
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通讯作者：
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作者：
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DOI：
10.1002/anie.199616971
发表时间：
1996-08-19
期刊：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH
影响因子：
--
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通讯作者：
Sontag, B
DOI：
10.1073/pnas.0904897106
发表时间：
2009-08-25
影响因子：
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Stehle, Thilo
影响因子：
3.2
作者：
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通讯作者：
Wessjohann, Ludger