Effects of heat treatment and simulated digestion on the properties and osteogenic activity of bovine lactoferrin
Effects of heat treatment and simulated digestion on the properties and osteogenic activity of bovine lactoferrin
复制标题
热处理和模拟消化对牛乳铁蛋白性质和成骨活性的影响
DOI：
10.1016/j.lwt.2022.113475
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发表时间：
2022-06
期刊：
影响因子：
--
通讯作者：
Huiyuan Guo
中科院分区：
文献类型：
--
作者：
Yao Lu;Yixuan Li;Yingying Lin;Jingxuan Wang;Mengjuan Ma;Huiyuan Guo
已知乳铁蛋白（LF）可刺激成骨细胞增殖和分化，有可能应用于改善骨骼健康的产品中。但LF对热处理敏感，口服给药后在胃肠道内发生水解，可能影响其成骨活性。为了确定热处理的影响，我们探索了在乳制品生产中常用的四种条件下牛LF的二级结构和表面疏水性的变化以及聚集沿着的表面电荷变化，发现72 °C/15 s，带来最小的结构变化，是保持LF促进成骨作用的最佳条件，而LF在85 °C/10 min和95 °C/10 min处理时完全丧失了这种作用。建立了体外成人和婴儿消化模型来鉴定LF的降解，尽管LF对成骨细胞生长的作用受损，肠消化期的LF水解物具有较强的促进成骨细胞分化的作用，不依赖于p38和维生素D受体。
Lactoferrin (LF) is known to stimulate osteoblast proliferation and differentiation, potential to be applied in products improving skeletal health. However, LF is sensitive to thermal processing, and oral administration leads to hydrolysis in gastrointestinal tract, probably influencing the osteogenic activity. To determine the impacts of heat treatment, we explored the changes in secondary structure and surface hydrophobicity underlying aggregation along with surface charge alteration of bovine LF under four conditions commonly used in dairy production and found 72 °C/15 s, bringing the least structural alteration, was optimal to retain effect of LF promoting osteogenesis, while LF was completely deprived of the effect treated at 85 °C/10 min and 95 °C/10 min.In vitroadult and infant digestion models were established to identify the degradation of LF, and, despite impaired effect on osteoblast growth, LF hydrolysates from intestinal digestion stage presented stronger effect of boosting osteoblast differentiation independent of p38 and vitamin D receptor.
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DOI：
10.1111/j.1651-2227.1982.tb09560.x
发表时间：
1982-11
期刊：
Acta Pædiatrica
影响因子：
--
作者：
G. Spik;B. Brunet;C. Mazurier-Dehaine;G. Fontaine;J. Montreuil
通讯作者：
G. Spik;B. Brunet;C. Mazurier-Dehaine;G. Fontaine;J. Montreuil
影响因子：
4.8
作者：
Olsson, N;Piek, E;Nilsson, G
通讯作者：
Nilsson, G
影响因子：
6
作者：
Liu, Jinfang;Yang, Jie;Gao, Yanxiang
通讯作者：
Gao, Yanxiang
影响因子：
0.8
作者：
Lizzi, A. R.;Carnicelli, V.;Amicosante, G.
通讯作者：
Amicosante, G.
影响因子：
6
作者：
Li, Quanyang;Lan, Haijing;Zhao, Zhengtao
通讯作者：
Zhao, Zhengtao