Complex regulation by Apetala2 domain-containing transcription factors revealed through analysis of the stress-responsive TdCor410b promoter from durum wheat.
Complex regulation by Apetala2 domain-containing transcription factors revealed through analysis of the stress-responsive TdCor410b promoter from durum wheat.
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DOI：
10.1371/journal.pone.0058713
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Lopato S
中科院分区：
文献类型：
--
作者：
Eini O;Yang N;Pyvovarenko T;Pillman K;Bazanova N;Tikhomirov N;Eliby S;Shirley N;Sivasankar S;Tingey S;Langridge P;Hrmova M;Lopato S
当暴露于寒冷、干旱和受伤等胁迫时，小麦脱水蛋白基因 Cor410b 的表达被诱导高于其非胁迫水平数倍。 TdCor410b 启动子的删除分析揭示了单个功能性 C 重复 (CRT) 元件。使用小麦和大麦 cDNA 文库的酵母单杂交筛选，显示 7 个转录因子 (TF) 与该 CRT 元件结合，其中只有一个属于 DREB 类 TF。其余六个编码的乙烯反应因子 (ERF) 属于三个不同的亚家族。对这些 TF 的结合选择性分析表明，所有 7 个都可以与 CRT 元件 (GCCGAC) 结合，并且 6 个 ERF 中的 3 个可以与 CRT 元件和乙烯响应性 GCC-box (GCCGCC) 结合。 TaERF4 亚家族成员特异性结合 CRT 元件，并且不结合 GCC-box 或 DRE 元件 (ACCGAC)。分子建模和定点诱变鉴定出 TaERF4 样蛋白的 Apetala2 (AP2) 结构域中的单个残基 Pro42，该残基在单子叶植物中保守，负责该亚家族的识别选择性。我们认为 DREB 和 ERF 蛋白均通过单个关键 CRT 元件调节 Cor410b 基因的表达。 TaERF4 亚家族的成员是 Cor410b 基因表达的特异性正调节因子。
Expression of the wheat dehydrin gene Cor410b is induced several fold above its non-stressed levels upon exposure to stresses such as cold, drought and wounding. Deletion analysis of the TdCor410b promoter revealed a single functional C-repeat (CRT) element. Seven transcription factors (TFs) were shown to bind to this CRT element using yeast one-hybrid screens of wheat and barley cDNA libraries, of which only one belonged to the DREB class of TFs. The remaining six encoded ethylene response factors (ERFs) belong to three separate subfamilies. Analysis of binding selectivity of these TFs indicated that all seven could bind to the CRT element (GCCGAC), and that three of the six ERFs could bind both to the CRT element and the ethylene-responsive GCC-box (GCCGCC). The TaERF4 subfamily members specifically bound the CRT element, and did not bind either the GCC-box or DRE element (ACCGAC). Molecular modeling and site-directed mutagenesis identified a single residue Pro42 in the Apetala2 (AP2) domain of TaERF4-like proteins that is conserved in monocotyledonous plants and is responsible for the recognition selectivity of this subfamily. We suggest that both DREB and ERF proteins regulate expression of the Cor410b gene through a single, critical CRT element. Members of the TaERF4 subfamily are specific, positive regulators of Cor410b gene expression.
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影响因子：
5.1
作者：
Gao, MJ;Allard, G;Singh, J
通讯作者：
Singh, J
DOI：
10.1073/pnas.94.11.5961
发表时间：
1997-05-27
影响因子：
11.1
作者：
Buttner, M;Singh, KB
通讯作者：
Singh, KB
DOI：
10.1002/prot.340230412
发表时间：
1995-12-01
期刊：
PROTEINS-STRUCTURE FUNCTION AND GENETICS
影响因子：
--
作者：
Frishman, D;Argos, P
通讯作者：
Argos, P
影响因子：
7.3
作者：
Cao, Wan-Hong;Liu, Jun;Chen, Shou-Yi
通讯作者：
Chen, Shou-Yi
影响因子：
5.2
作者：
GROSSI, M;GULLI, M;CATTIVELLI, L
通讯作者：
CATTIVELLI, L