Models of lineage switching in hematopoietic development: a new myeloid-committed eosinophil cell line (YJ) demonstrates trilineage potential
Models of lineage switching in hematopoietic development: a new myeloid-committed eosinophil cell line (YJ) demonstrates trilineage potential
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造血发育中的谱系转换模型：一种新的骨髓定向嗜酸性粒细胞系（YJ）表现出三谱系潜力
DOI：
10.1038/sj.leu.2401115
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发表时间：
1998
期刊：
影响因子：
11.4
通讯作者：
Toshio Suda
中科院分区：
文献类型：
--
作者：
Yuji Yamaguchi;Hitoshi Nishio;Tadashi Kasahara;Steven J. Ackerman;H. Koyanagi;Toshio Suda
从1例伴有嗜酸性粒细胞增多症的慢性粒单核细胞白血病(CMMoL)患者的外周血细胞中建立了一株新的人类白血病细胞系，命名为YJ。在含10%胎牛血清的RPMI1640培养液中，YJ细胞大部分为成髓细胞，少数细胞有嗜酸性颗粒。YJ细胞表面标志CD33阳性，CD34、CD16、CD23阴性。通过快速绿/中性红组织化学染色和嗜酸性粒细胞相关颗粒蛋白(ECP)、嗜酸性粒细胞衍生神经毒素(EDN)、嗜酸性细胞过氧化物酶(EPO)和主要碱性蛋白(MBP)的mRNAs表达，以及Charcot-Leyden晶体(CLC)蛋白的表达，证实YJ细胞的嗜酸性特征。佛波酯(PMA)可诱导YJ细胞向单核细胞分化。α-丁酸萘酯酶染色形态分析、CD14表达及Egr-1mRNA表达增强证实了PMA处理的YJ细胞具有单核细胞特性。全反式维甲酸(RA)可诱导YJ细胞向中性粒细胞方向分化。经2- μ-M-RA处理的YJ细胞在体外可分化为中性粒细胞和带状中性粒细胞，并可使NBT阳性细胞数增加，gp91Phox基因表达增强。因此，YJ细胞株表现出嗜酸粒细胞的特征，但能够分化为单核细胞或中性粒细胞分别对PMA或RA的反应。Northern印迹分析检测髓系分化相关转录因子基因的表达。随着巨噬细胞的分化，Egr-1的表达增加。中性粒细胞分化后，C/EBPmRNA和MZF-1mRNA表达增加。YJ细胞系对于阐明嗜酸性粒细胞向单核细胞或中性粒细胞谱系转换的分子机制是有用的。
A new human leukemia cell line with an eosinophilic phenotype, designated YJ, was established from the peripheral blood cells of a patient with chronic myelomonocytic leukemia (CMMoL) with eosinophilia. When cultured in RPMI 1640 medium containing 10% fetal bovine serum, most YJ cells were myeloblastoid with a small number of the cells having eosinophilic granules. Cell surface markers in the YJ cells were positive for CD33 and were negative for CD34, CD16 and CD23. The eosinophilic characteristics of YJ cells were confirmed by histochemical staining with Fast-Green/Neutral-Red and by the expression of mRNAs for eosinophil-associated granule proteins, eosinophil cationic protein (ECP), eosinophil-derived neurotoxin (EDN), eosinophil peroxidase (EPO), and major basic protein (MBP), and for the Charcot–Leyden crystal (CLC) protein. The YJ cells could be induced towards monocytic differentiation by stimulation with phorbol 12-myristate 13-acetate (PMA). The monocytic characteristics of YJ cells treated with PMA were confirmed by morphological analysis with α-naphthyl butyrate esterase staining, by CD14 expression, and by increased expression of Egr-1 mRNA. Furthermore, YJ cells could be differentiated towards the neutrophil lineage by stimulation with all-trans retinoic acid (RA). YJ cells treated in vitro with 2 μM RA differentiated into metamyelocytes and band neutrophils, and increased the number of nitroblue tetrazolium (NBT)-positive cells and increased gp91phox mRNA expression. Thus, the YJ cell line exhibited eosinophilic characteristics, but was able to differentiate to the monocytic or neutrophilic lineages in response to PMA or RA, respectively. The expression of genes for transcription factors involved in myeloid differentiation was evaluated by Northern blot analysis. Increased expression of Egr-1 was observed with macrophage differentiation. In contrast, increased expressions of C/EBPβ and MZF-1 mRNA occurred with neutrophilic differentiation. The YJ cell line should be useful for elucidating the molecular mechanisms governing lineage switching from the eosinophil to monocytic or neutrophil lineages.
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嗜酸性粒细胞主要碱性蛋白诱导人嗜酸性粒细胞脱粒和产生 IL-8。
DOI：
--
发表时间：
1995
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Kita,H;Abu-Ghazaleh,RI;Sur,S;Gleich,GJ
通讯作者：
Gleich,GJ
正常外周血嗜酸性粒细胞持续产生 IL-4 和 IL-10，并刺激产生 IL-8。
DOI：
--
发表时间：
1996
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Nakajima,H;Gleich,GJ;Kita,H
通讯作者：
Kita,H
从人早幼粒细胞白血病 (HL-60) 细胞建立嗜酸性亚系：证明 HL-60 干细胞的多能性和单谱系定型。
影响因子：
20.3
作者：
Tomonaga,M;Gasson,JC;Quan,SG;Golde,DW
通讯作者：
Golde,DW
人嗜酸性粒细胞衍生的神经毒素的分子克隆：核糖核酸酶基因家族的成员。
DOI：
10.1073/pnas.86.12.4460
发表时间：
1989
影响因子：
11.1
作者：
Rosenberg,HF;Tenen,DG;Ackerman,SJ
通讯作者：
Ackerman,SJ
影响因子：
20.3
作者：
CHEN, HM;ZHANG, P;TENEN, DG
通讯作者：
TENEN, DG