Carbon Nanoparticles Inhibit the Aggregation of Prion Protein as Revealed by Experiments and Atomistic Simulations
Carbon Nanoparticles Inhibit the Aggregation of Prion Protein as Revealed by Experiments and Atomistic Simulations
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实验和原子模拟表明碳纳米颗粒可抑制朊病毒蛋白的聚集
DOI：
10.1021/acs.jcim.8b00725
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发表时间：
2019-01
影响因子：
5.6
通讯作者：
Liu Huanxiang
中科院分区：
文献类型：
--
作者：
Zhou Shuangyan;Zhu Yongchang;Yao Xiaojun;Liu Huanxiang
碳纳米颗粒（NPs）的特殊性质在生物技术和生物医学中的应用引起了极大的关注，例如，淀粉样变性病领域的研究迄今为止，碳纳米颗粒是否会促进或抑制淀粉样蛋白原纤维的形成仍不清楚。在这里，为了揭示包括石墨烯和碳纳米管的碳纳米颗粒（NP）对朊病毒蛋白聚集的影响，其错误折叠和聚集将导致朊病毒疾病，进行了ThT荧光测定和分子动力学（MD）模拟。ThT荧光分析表明，石墨烯和碳纳米管都可以抑制朊病毒蛋白的原纤维形成，尤其是石墨烯。进一步对PrP 127 -147四聚体进行的分子动力学模拟表明，朊病毒蛋白和碳纳米颗粒之间的相互作用通过减少肽间相互作用降低了PrP 127 -147的聚集趋势，从而抑制了β折叠的形成。同时，芳香残基通过π-π堆积作用对碳纳米粒子的抑制作用有很大贡献。所得结果可增加我们对纳米颗粒与淀粉样蛋白相关蛋白之间相互作用的理解。
The specific properties of carbon nanoparticles (NPs) have attracted great attention in applications in biotechnology and biomedicine, e.g., in the field of amyloidosis. To date, it is still indefinable whether carbon NPs would promote or inhibit the fibril formation of amyloid proteins. Here, to uncover the effects of carbon nanoparticles (NPs) including graphene and carbon nanotubes on the aggregation of prion proteins, whose misfolding and aggregation will lead to prion diseases, a ThT fluorescence assay and a molecular dynamics (MD) simulation were performed. The ThT fluorescence assay reveals that both graphene and carbon nanotubes can inhibit the fibril formation of prion proteins, especially graphene. Further MD simulation of the PrP127-147 tetramer with or without carbon NPs suggests that the interactions between prion proteins and carbon NPs reduce the aggregation tendency of PrP127-147 by decreasing the interpeptide interactions and thus inhibiting β-sheet formation. Meanwhile, aromatic residues greatly contribute to the inhibition effects of carbon NPs by a π-π stacking interaction. The obtained results can increase our understanding on the interaction between nanoparticles and amyloid-related proteins.
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影响因子：
4.9
作者：
F. Re;Maria Gregori;M. Masserini
通讯作者：
F. Re;Maria Gregori;M. Masserini
DOI：
10.1073/pnas.0712036105
发表时间：
2008-02
期刊：
Proceedings of the National Academy of Sciences
影响因子：
--
作者：
J. Stöhr;N. Weinmann;H. Wille;T. Kaimann;L. Nagel-Steger;E. Birkmann;G. Panza;S. Prusiner;M. Eigen;D. Riesner
通讯作者：
J. Stöhr;N. Weinmann;H. Wille;T. Kaimann;L. Nagel-Steger;E. Birkmann;G. Panza;S. Prusiner;M. Eigen;D. Riesner
DOI：
10.1002/prot.340230412
发表时间：
1995-12-01
期刊：
PROTEINS-STRUCTURE FUNCTION AND GENETICS
影响因子：
--
作者：
Frishman, D;Argos, P
通讯作者：
Argos, P
影响因子：
--
作者：
Ni-Shian Lin;John Chin Hao Chao;Hsin-Mei Cheng;Fang-Chieh Chou;Chi-Fon Chang;Yun-Ru Chen;Yu-Jen Chang;Shing‐Jong Huang;J. Chan
通讯作者：
Ni-Shian Lin;John Chin Hao Chao;Hsin-Mei Cheng;Fang-Chieh Chou;Chi-Fon Chang;Yun-Ru Chen;Yu-Jen Chang;Shing‐Jong Huang;J. Chan
DOI：
10.1073/pnas.0701250104
发表时间：
2007-05-22
影响因子：
11.1
作者：
Linse, Sara;Cabaleiro-Lago, Celia;Dawson, Kenneth A.
通讯作者：
Dawson, Kenneth A.