Reversible inactivation of cell-type-specific regulatory and structural genes in migrating isolated striated muscle cells of jellyfish.
Reversible inactivation of cell-type-specific regulatory and structural genes in migrating isolated striated muscle cells of jellyfish.
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水母迁移中分离的横纹肌细胞中细胞类型特异性调节和结构基因的可逆失活。
DOI：
10.1006/dbio.1999.9347
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发表时间：
1999
影响因子：
2.7
通讯作者：
V. Schmid
中科院分区：
文献类型：
--
作者：
N. Yanze;H. Gröger;P. Müller;V. Schmid
我们已经调查，通过RT-PCR和原位杂交，表达编码调控和结构蛋白的基因在迁移单核横纹肌细胞的水母Podocoryne carnea。三个同源异型盒基因Otx，Cnox 1-Pc和Cnox 3-Pc的表达;肌球蛋白重链基因（Myo 1）的特异性剪接变体;和原肌球蛋白（Tpm 2）在分离和培养的横纹肌组织中是稳定的。当移植到无细胞的细胞外基质（ECM）上时，组织片段的肌细胞离开其天然ECM并作为连贯组织迁移到宿主ECM上，直到形成拉伸的细胞单层。在移植分离物的第一个细胞与宿主ECM接触后不久，Otx和Cnox 1-Pc表达在移植物的所有细胞中完全关闭，包括那些仍然粘附在其天然ECM上的细胞。Myo 1信息延迟消失，而Tpm 2的表达水平强烈降低。然而，同源框基因Cnox 3-Pc（一种msh样基因）和广泛表达的延伸因子1 α的表达不受迁移过程的影响。一旦细胞迁移停止，所有基因在12-24小时后重新表达。我们的研究结果表明，最初的几个迁移细胞诱导基因表达的变化，这是迅速沟通整个组织。此外，我们发现，尽管细胞类型特异性调控和结构基因的瞬时失活，横纹肌细胞的承诺仍然保持稳定。
We have investigated, by RT-PCR and in situ hybridization, expression of genes encoding regulatory and structural proteins in migrating mononucleated striated muscle cells of the medusa Podocoryne carnea. Expression of the three homeobox genes Otx, Cnox1-Pc, and Cnox3-Pc; a specific splice variant of the myosin heavy chain gene (Myo1); and a tropomyosin (Tpm2) is stable in isolated and cultured striated muscle tissue. When grafted onto cell-free extracellular matrix (ECM), muscle cells of the tissue fragments leave their native ECM and migrate as a coherent tissue onto a host ECM until a stretched cell monolayer is formed. Shortly after the first cells of the grafted isolate have made contact with the host ECM, Otx and Cnox1-Pc expression is completely turned off in all cells of the graft, including those still adhering to their native ECM. Myo1 message disappears with a delay while the expression level of Tpm2 is strongly reduced. However, expression of the homeobox gene Cnox3-Pc, a msh-like gene, and of the ubiquitously expressed elongation factor 1 alpha is not affected by the migration process. All genes are reexpressed after 12-24 h, once migration of the cells has ceased. Our results demonstrate that the first few migrating cells induce a change in gene expression which is rapidly communicated throughout the entire tissue. Furthermore, we showed that commitment of striated muscle cells remains stable despite the transient inactivation of cell-type-specific regulatory and structural genes.
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影响因子：
56.9
作者：
Kheradmand, F;Werner, E;Werb, Z
通讯作者：
Werb, Z
影响因子：
7.5
作者：
Boudreau, N;Bissell, MJ
通讯作者：
Bissell, MJ
DOI：
--
发表时间：
1993-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
K. Vuori;E. Ruoslahti
通讯作者：
K. Vuori;E. Ruoslahti
DOI：
10.1073/pnas.94.25.13661
发表时间：
1997-12-09
影响因子：
11.1
作者：
Pelham, RJ;Wang, YL
通讯作者：
Wang, YL