Analysis of gene expression profiling variations induced by hsa‑miR‑145‑5p‑overexpression in laryngeal squamous cell carcinoma cell line Tu‑177.
Analysis of gene expression profiling variations induced by hsa‑miR‑145‑5p‑overexpression in laryngeal squamous cell carcinoma cell line Tu‑177.
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喉鳞状细胞癌细胞系Tu-177 hsa-miR-145-5p过表达诱导的基因表达谱变异分析
DOI：
10.3892/mmr.2017.7360
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发表时间：
2017-11
影响因子：
3.4
通讯作者：
Wang B
中科院分区：
文献类型：
--
作者：
Ding Y;Wu Y;Gao W;Zhang C;Zhao Q;Guo H;Qu X;Wen S;Wang B
本研究旨在探讨hsa-miR-145-5p对喉鳞状细胞癌(LSCC)细胞系Tu-177基因网络和生物学功能的影响。建立hsa-miR-145-5p过表达的Tu-177细胞模型，分析miR-145-5p过表达细胞和阴性对照(NC)细胞的基因表达芯片数据。对两组间的差异表达基因(Deg)进行鉴定，并通过功能丰富分析预测其潜在功能。此外，从DEGS中鉴定了miR-145-5P的靶标，并分析了它们的潜在功能和蛋白质-蛋白质相互作用(PPI)。用定量聚合酶链式反应分析乙酰辅酶A羧基酶β、成纤维细胞生长因子受体1、蛋白磷酸酶3催化亚基a和脾相关酪氨酸激酶的基因表达。与NC细胞相比，hsa-miR-145-5p过表达的Tu-177细胞共有1,501个上调基因和887个下调基因。在这些DEG中，164个上调基因和221个下调基因被预测为hsa-miR-145-5p的靶点。上调的靶基因主要与免疫功能有关，而下调的靶基因在P53信号通路中显著丰富。在由267个靶基因组成的PPI网络中，ACACB上调的程度最大，除了FGFR1等上调的基因外，还与PPP3CA和SYK等下调的基因相互作用。在miR-145-5p过表达的Tu-177细胞中，ACACB和FGFR1的mRNA表达显著增强，而miR-145-5p过表达显著降低PPP3CA和SYK的mRNA表达。HSA-miR-145-5p可能通过调节细胞增殖和侵袭、脂肪酸代谢、免疫和P53信号通路等多个细胞过程在喉癌中发挥抗癌作用。这些发现为进一步研究miR-145-5p在喉癌中的功能提供了新的信息。
The present study aimed to investigate the variations of the gene network and biological functions induced by hsa-miR-145-5p in the laryngeal squamous cell carcinoma (LSCC) cell line Tu-177. A hsa-miR-145-5p-overexpressed Tu-177 cell model was established, and the gene expression microarray data of miR-145-5p-overexpressed cells and negative control (NC) cells were analyzed. The differentially expressed genes (DEGs) between two groups were identified, and their potential functions were predicted by functional enrichment analysis. Furthermore, the targets of miR-145-5p were identified from the DEGs, and their potential functions and protein-protein interactions (PPIs) were analyzed. The mRNA expressions of acetyl-CoA carboxylase β (ACACB), fibroblast growth factor receptor 1 (FGFR1), protein phosphatase 3 catalytic subunit a (PPP3CA) and spleen associated tyrosine kinase (SYK), were analyzed via quantitative polymerase chain reaction. A total of 1,501 upregulated and 887 downregulated genes were identified in the hsa-miR-145-5p-overexpressed Tu-177 cells, compared with the NC cells. Of these DEGs, 164 upregulated and 221 downregulated genes were predicted to be targeted by hsa-miR-145-5p. The upregulated target genes were primarily associated with functions of immunity, whereas the downregulated target genes were significantly enriched in the p53 signaling pathway. In the PPI network consisting of 267 target genes, the upregulated ACACB had the greatest degree and interacted with downregulated genes including PPP3CA and SYK, in addition to upregulated genes, including FGFR1. The mRNA expressions of ACACB and FGFR1were markedly enhanced in miR-145-5p-overexpressed Tu-177 cells, whereas overexpressing miR-145-5p significantly reduced mRNA expression of PPP3CA and SYK. hsa-miR-145-5p may exhibit an anticancer role in LSCC via regulating multiple cell processes, including cell proliferation and invasion, fatty acid metabolism, immunity and p53 signaling pathway. These findings provide novel information for the future investigation of miR-145-5p functions in LSCC.
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影响因子：
--
作者：
Nersisyan L;Samsonyan R;Arakelyan A
通讯作者：
Arakelyan A
影响因子：
3.1
作者：
Mak, Lora;Liggi, Sonia;Kirchmair, Johannes
通讯作者：
Kirchmair, Johannes
影响因子：
11.2
作者：
Bergmann, Christoph;Strauss, Laura;Whiteside, Theresa L.
通讯作者：
Whiteside, Theresa L.
影响因子：
64.5
作者：
Lewis, BP;Shih, IH;Burge, CB
通讯作者：
Burge, CB
DOI：
10.1093/bioinformatics/btp101
发表时间：
2009-04-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Bindea G;Mlecnik B;Hackl H;Charoentong P;Tosolini M;Kirilovsky A;Fridman WH;Pagès F;Trajanoski Z;Galon J
通讯作者：
Galon J