Development and validation of an animal model of prostate inflammation-induced chronic pelvic pain: evaluating from inflammation of the prostate to pain behavioral modifications.
Development and validation of an animal model of prostate inflammation-induced chronic pelvic pain: evaluating from inflammation of the prostate to pain behavioral modifications.
复制标题
前列腺炎症引起的慢性盆腔疼痛动物模型的开发和验证：从前列腺炎症到疼痛行为改变的评估
DOI：
10.1371/journal.pone.0096824
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Zeng F;Chen H;Yang J;Wang L;Cui Y;Guan X;Wang Z;Niu J;Zu X;Qi L;Zhang X;Tang Z;Liu L
背景 慢性前列腺炎/慢性盆腔疼痛综合征（CP/CPPS）是最常见的前列腺炎类型。部分由于缺乏合适的动物模型，该病的发病机制尚不清楚。在当前的研究中，我们开发并验证了一种动物模型，用于通过前列腺内注射 λ-角叉菜胶来治疗非细菌性前列腺炎和前列腺炎症引起的大鼠慢性盆腔疼痛。方法采用体重250~350 g的雄性Sprague-Dawley大鼠进行实验。前列腺内注射3%λ-卡拉胶后，在不同时间点（注射后24小时、7天、14天和30天），将辐射热和冯弗雷丝施加于大鼠阴囊，分别测量热阈值和机械阈值。然后取出前列腺进行组织学检查，并通过Western-blot测定环氧合酶（COX）2蛋白表达。静脉注射伊文思蓝(50 mg/kg)以评估注射λ-卡拉胶后不同时间点的血浆蛋白外渗情况。结果与对照组相比，发炎动物在24小时和7天时表现出机械阈值（机械异常性疼痛）显着降低（分别为p = 0.022、0.046），在24小时、7天和14天时表现出热阈值（热痛觉过敏）显着降低（分别为p = 0.014、0.018、0.002）。阴囊皮肤。注射后24小时、7天和14天观察到炎症细胞积累、COX2表达和伊文思蓝外渗显着增加。结论 前列腺内注射λ-卡拉胶可诱发神经源性前列腺炎及前列腺炎性疼痛，且持续至少2周。目前的模型有望成为研究男性慢性盆腔疼痛的神经生物学机制的有价值的临床前工具。
Background Chronic prostatitis/Chronic pelvic pain syndrome (CP/CPPS) is the most common type of prostatitis. Due to the lack of a suitable animal model partly, the pathogenesis for this condition is obscure. In the current study we developed and validated an animal model for nonbacterial prostatitis and prostate inflammation-induced chronic pelvic pain in rats with the use of intraprostatic injection of λ-carrageenan. Methods Male Sprague-Dawley rats weighing 250–350 g were used for the experiments. After intraprostatic injection of 3% λ-carrageenan, at different time points(after 24 h, 7d, 14d and 30d of injection), radiant heat and von Frey filaments were applied to the scrotum of rats to measure the heat and mechanical thresholds respectively. Then the prostate was removed for histology, and cyclooxygenase (COX) 2 protein expression was determined by Western-blot. Evans blue(50 mg/kg) was also injected intravenously to assess for plasma protein extravasation at different time points after injection of λ-carrageenan. Results Compared to control group, inflamed animals showed a significant reduction in mechanical threshold (mechanical allodynia) at 24 h and 7d(p = 0.022,0.046, respectively), and a significant reduction in heat threshold (thermal hyperalgesia) at 24 h, 7d and 14d(p = 0.014, 0.018, 0.002, respectively) in the scrotal skin. Significant increase of inflammatory cell accumulation,COX2 expression and Evans blue extravasation were observed at 24 h, 7d and 14d after injection. Conclusions Intraprostatic λ-carrageenan injection induced neurogenic prostatitis and prostate inflammation pain, which lasted at least 2 weeks. The current model is expected to be a valuable preclinical tool to study the neurobiological mechanisms of male chronic pelvic pain.
登录
查看更多内容
影响因子：
2.9
作者：
Liu, Long-Fei;Yang, Jin-Rui;Yang, Tu-Bao
通讯作者：
Yang, Tu-Bao
影响因子：
4.5
作者：
Schneider, H;Wilbrandt, K;Weidner, W
通讯作者：
Weidner, W
影响因子：
6.6
作者：
Nickel, J. Curtis;Roehrborn, Claus G.;Rittmaster, Roger S.
通讯作者：
Rittmaster, Roger S.
影响因子：
7.4
作者：
Radhakrishnan, R;Moore, SA;Sluka, KA
通讯作者：
Sluka, KA
影响因子：
23.4
作者：
Chuang, Yao-Chi;Yoshimura, Naoki;Chancellor, Michael B.
通讯作者：
Chancellor, Michael B.