Tumor necrosis factor improves vascularization in osteogenic grafts engineered with human adipose-derived stem/stromal cells.
Tumor necrosis factor improves vascularization in osteogenic grafts engineered with human adipose-derived stem/stromal cells.
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DOI：
10.1371/journal.pone.0107199
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Grayson WL
中科院分区：
文献类型：
--
作者：
Hutton DL;Kondragunta R;Moore EM;Hung BP;Jia X;Grayson WL
The innate immune response following bone injury plays an important role in promoting cellular recruitment, revascularization, and other repair mechanisms. Tumor necrosis factor-α (TNF) is a prominent pro-inflammatory cytokine in this cascade, and has been previously shown to improve bone formation and angiogenesis in a dose- and timing-dependent manner. This ability to positively impact both osteogenesis and vascular growth may benefit bone tissue engineering, as vasculature is essential to maintaining cell viability in large grafts after implantation. Here, we investigated the effects of exogenous TNF on the induction of adipose-derived stem/stromal cells (ASCs) to engineer pre-vascularized osteogenic tissue in vitro with respect to dose, timing, and co-stimulation with other inflammatory mediators. We found that acute (2-day), low-dose exposure to TNF promoted vascularization, whereas higher doses and continuous exposure inhibited vascular growth. Co-stimulation with platelet-derived growth factor (PDGF), another key factor released following bone injury, increased vascular network formation synergistically with TNF. ASC-seeded grafts were then cultured within polycaprolactone-fibrin composite scaffolds and implanted in nude rats for 2 weeks, resulting in further tissue maturation and increased angiogenic ingrowth in TNF-treated grafts. VEGF-A expression levels were significantly higher in TNF-treated grafts immediately prior to implantation, indicating a long-term pro-angiogenic effect. These findings demonstrate that TNF has the potential to promote vasculogenesis in engineered osteogenic grafts both in vitro and in vivo. Thus, modulation and/or recapitulation of the immune response following bone injury may be a beneficial strategy for bone tissue engineering.
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DOI：
10.1080/09205063.2013.803455
发表时间：
2013
期刊：
Journal of biomaterials science. Polymer edition
影响因子：
--
作者：
Mountziaris PM;Dennis Lehman E;Mountziaris I;Sing DC;Kasper FK;Mikos AG
通讯作者：
Mikos AG
影响因子：
8.7
作者：
Nakagami, H;Cui, TX;Horiuchi, M
通讯作者：
Horiuchi, M
DOI：
10.1073/pnas.1018501108
发表时间：
2011-01-25
影响因子：
11.1
作者：
Glass, Graeme E.;Chan, James K.;Nanchahal, Jagdeep
通讯作者：
Nanchahal, Jagdeep
影响因子：
4
作者：
Lu, ZuFu;Wang, Guocheng;Zreiqat, Hala
通讯作者：
Zreiqat, Hala
影响因子：
6.4
作者：
Kolar, Paula;Schmidt-Bleek, Katharina;Duda, Georg N.
通讯作者：
Duda, Georg N.