Blood level and brain distribution of thyrotropin-releasing hormone (TRH) determined by radioimmunoassay after intravenous administration in rats.
Blood level and brain distribution of thyrotropin-releasing hormone (TRH) determined by radioimmunoassay after intravenous administration in rats.
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放射免疫法测定大鼠静脉注射后促甲状腺素释放激素（TRH）的血浓度和脑分布。
DOI：
10.1248/bpb1978.3.500
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
N. Nihei
中科院分区：
文献类型：
--
作者：
Y. Nagai;S. Yokohama;Y. Nagawa;Y. Hirooka;N. Nihei
通过放射免疫测定法研究了大鼠静脉注射酒石酸TRH一水合物（TRH-T）后血液中免疫反应性TRH的水平和脑分布。研究发现，通过微波照射使大脑中 TRH 降解酶完全失活的最佳条件是 5kW 功率持续 1.5 秒。内源性TRH在下丘脑中浓度最高，其次是脑干和丘脑，在大脑皮层（包括海马和小脑）中浓度最低。以 0.5、5 和 25 mg/kg 剂量施用 TRH-T 后 TRH 血液水平的时程符合两室开放模型，β 相半衰期随剂量依赖性增加。所施用的TRH迅速从血液掺入全脑，并且在施用后1分钟达到对应于所施用总剂量的0.108--0.166%的峰值脑水平。大脑TRH被消除，半衰期约为3分钟。施用的TRH分布在整个大脑中，下丘脑浓度最高，其次是脑干、小脑、丘脑和大脑皮层。 TRH 往往从小脑中消除得最快。
The blood level and brain distribution of immunoreactive TRH after intravenous administration of TRH tartrate monohydrate (TRH-T) were investigated by radioimmunoassay in rats. Optimal condition for complete inactivation of TRH-degradative enzymes in brain by a microwave irradiation was found to be a 5kW power for 1.5 seconds. The endogenous TRH was present at the highest concentration in the hypothalamus, followed by the brain stem and thalamus, and was the lowest in the cerebral cortex including hippocampus and cerebellum. The time-course of TRH blood levels following administration of TRH-T at 0.5, 5 and 25 mg/kg fitted to a two compartment open model, and the half-lives in beta-phase increased dose-dependently. The administered TRH was incorporated rapidly from blood into whole brain, and the peak brain level corresponding to 0.108--0.166% of the total dose administered was attained 1 min after the administration. The brain TRH was eliminated with a half-life of about 3 min. The administered TRH was distributed throughout the brain, at the highest concentration in the hypothalamus, followed by the brain stem, cerebellum, thalamus and cerebral cortex. TRH tended to be eliminated most rapidly from the cerebellum.
七鳃鳗中枢神经系统中的促甲状腺素释放激素。
影响因子：
2.9
作者：
Youngs,LJ;Winokur,A;Selzer,ME
通讯作者：
Selzer,ME