ERFVII action and modulation through oxygen-sensing in Arabidopsis thaliana.
ERFVII action and modulation through oxygen-sensing in Arabidopsis thaliana.
复制标题
DOI：
10.1038/s41467-023-40366-y
复制
发表时间：
2023-08-03
影响因子：
16.6
通讯作者：
Holdsworth, Michael J.
中科院分区：
文献类型：
--
作者：
Zubrycka, Agata;Dambire, Charlene;Carbonare, Laura Dalle;Sharma, Gunjan;Boeckx, Tinne;Swarup, Kamal;Sturrock, Craig J.;Atkinson, Brian S.;Swarup, Ranjan;Corbineau, Francoise;Oldham, Neil J.;Holdsworth, Michael J.
氧是植物生物学的关键信号成分，虽然先前在拟南芥中描述了氧传感机制，但相关植物半胱氨酸氧化酶 (PCO) N-degron 途径和 VII 组乙烯响应因子 (ERFVII) 转录因子底物的关键特征仍未测试或未知。我们证明，ERFVII 表现出根部耐缺氧性的非自主激活，并且对于根系在土壤缺氧条件下的发育和生存至关重要。我们确定了 ERFVII 在控制基因表达方面的综合作用，并通过 PCO N-degron 途径定义了 ERFVII 蛋白酶体依赖性氧调节稳定性所需的遗传和环境成分。使用植物提取物，观察到 ERFVII 的意外氨基末端半胱氨酸磺酸氧化水平，表明该途径需要额外的酶活性。我们的结果提供了对这种氧传感机制的特性、功能和读数的全面了解，该机制是通过其在调节 ERFVII 稳定性中的作用来定义的。氧气对于植物生命至关重要。在这里，作者定义了植物氧传感机制的新功能和组件，提供了对传感生物化学和允许植物根在土壤中生存的生理反应的理解。
Oxygen is a key signalling component of plant biology, and whilst an oxygen-sensing mechanism was previously described in Arabidopsis thaliana, key features of the associated PLANT CYSTEINE OXIDASE (PCO) N-degron pathway and Group VII ETHYLENE RESPONSE FACTOR (ERFVII) transcription factor substrates remain untested or unknown. We demonstrate that ERFVIIs show non-autonomous activation of root hypoxia tolerance and are essential for root development and survival under oxygen limiting conditions in soil. We determine the combined effects of ERFVIIs in controlling gene expression and define genetic and environmental components required for proteasome-dependent oxygen-regulated stability of ERFVIIs through the PCO N-degron pathway. Using a plant extract, unexpected amino-terminal cysteine sulphonic acid oxidation level of ERFVIIs was observed, suggesting a requirement for additional enzymatic activity within the pathway. Our results provide a holistic understanding of the properties, functions and readouts of this oxygen-sensing mechanism defined through its role in modulating ERFVII stability. Oxygen is essential for plant life. Here the authors define new functions and components of the plant oxygen sensing mechanism providing an understanding of the biochemistry of sensing and physiological responses allowing plant roots to survive in the soil.
登录
查看更多内容
DOI：
10.1016/j.cub.2022.01.037
发表时间：
2022-03-28
期刊：
Current biology : CB
影响因子：
--
作者：
Barreto P;Dambire C;Sharma G;Vicente J;Osborne R;Yassitepe J;Gibbs DJ;Maia IG;Holdsworth MJ;Arruda P
通讯作者：
Arruda P
影响因子：
4.8
作者：
Davydov, IV;Varshavsky, A
通讯作者：
Varshavsky, A
DOI：
10.1073/pnas.1321892112
发表时间：
2014-12-30
影响因子：
11.1
作者：
Green, Peter T.;Harms, Kyle E.;Connell, Joseph H.
通讯作者：
Connell, Joseph H.
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
7.2
作者：
Bui, Liem T.;Shukla, Vinay;Giuntoli, Beatrice
通讯作者：
Giuntoli, Beatrice