Barley endosomal MONENSIN SENSITIVITY1 is a target of the powdery mildew effector CSEP0162 and plays a role in plant immunity.
Barley endosomal MONENSIN SENSITIVITY1 is a target of the powdery mildew effector CSEP0162 and plays a role in plant immunity.
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大麦内体Monensin敏感性1是白粉病效应子CSEP0162的靶标，在植物免疫中起作用。
DOI：
10.1093/jxb/erac403
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发表时间：
2023-01-01
影响因子：
6.9
通讯作者：
中科院分区：
文献类型：
--
作者：
吸器和生物营养菌丝周围形成的包裹以及过敏反应（HR）细胞死亡是植物对丝状病原体的重要免疫反应。在这项研究中，我们研究了可能导致受白粉病真菌侵袭的易感大麦中缺乏这些反应的成分。我们发现这种病原体的效应子 CSEP0162 靶向植物莫能菌素 SENSITIVITY1 (MON1)，这对于多泡体与其靶膜的融合很重要。 CSEP0162 的过度表达和大麦 MON1 的沉默都会抑制包膜的形成。我们发现拟南芥生态型 No-0 对白粉病具有抗性，这部分依赖于 MON1。令人惊讶的是，我们发现 No-0 中 MON1 依赖性抵抗不仅包括包裹反应，还包括有效的 HR。同样，大麦中 MON1 的沉默也会阻断 Mla3 介导的基于 HR 的白粉病抗性。我们的结果表明 MON1 是重要的植物免疫成分，我们推测大麦白粉病真菌引入效应子 CSEP0162 来靶向 MON1，从而减少包膜形成和 HR。 MON1 对于激活 Rab7 样 GTP 酶至关重要，并且是大麦白粉病效应子 CSEP0162 的靶标，因此在植物免疫中发挥着重要作用。
Encasements formed around haustoria and biotrophic hyphae as well as hypersensitive reaction (HR) cell death are essential plant immune responses to filamentous pathogens. In this study we examine the components that may contribute to the absence of these responses in susceptible barley attacked by the powdery mildew fungus. We find that the effector CSEP0162 from this pathogen targets plant MONENSIN SENSITIVITY1 (MON1), which is important for the fusion of multivesicular bodies to their target membranes. Overexpression of CSEP0162 and silencing of barley MON1 both inhibit encasement formation. We find that the Arabidopsis ecotype No-0 has resistance to powdery mildew, and that this is partially dependent on MON1. Surprisingly, we find the MON1-dependent resistance in No-0 not only includes an encasement response, but also an effective HR. Similarly, silencing of MON1 in barley also blocks Mla3-mediated HR-based powdery mildew resistance. Our results indicate that MON1 is a vital plant immunity component, and we speculate that the barley powdery mildew fungus introduces the effector CSEP0162 to target MON1 and hence reduce encasement formation and HR. MON1 is essential for activation of Rab7-like GTPases and it is targeted by the barley powdery mildew effector CSEP0162, thus giving it an important role in plant immunity.
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影响因子：
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作者：
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