Minocycline inhibits the enhancement of antidromic primary afferent stimulation-evoked vasodilation following intradermal capsaicin injection.
Minocycline inhibits the enhancement of antidromic primary afferent stimulation-evoked vasodilation following intradermal capsaicin injection.
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DOI：
10.1016/j.neulet.2010.07.031
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发表时间：
2010-09-27
影响因子：
2.5
通讯作者：
Lin Q
中科院分区：
文献类型：
--
作者：
Gong K;Yue Y;Zou X;Li D;Lin Q
神经源性炎症由初级传入伤害感受器在外周组织中释放的炎症介质诱导。我们以前的研究表明，辣椒素皮内注射引起的神经源性炎症的结果从背根反射（DRR），其中涉及背根神经节（DRG）神经元的逆向激活的增强。许多研究已经报道了神经胶质细胞调节在疼痛中的重要作用。然而，目前尚不清楚胶质细胞是否参与神经源性炎症引起的疼痛的过程。在这里，我们测试的DRG卫星神经胶质细胞（SGCs）在这一过程中的作用，在麻醉大鼠通过管理的胶质细胞抑制剂，米诺环素。电刺激（ES，频率10 Hz;持续时间1 ms;强度3 mA）施加到L4-5背根的切断远端。刺激诱发旨在模拟DRR的逆行动作电位。使用多普勒流量计测量后爪中的局部皮肤血流量。逆向ES 10分钟引起了显着的血管舒张，可以抑制剂量依赖性的局部给药的降钙素基因相关肽受体拮抗剂，CGRP 8 -37。在ES前2 h，足爪皮内注射辣椒素，可显著增强逆向ES引起的血管舒张作用，这种增强作用可被米诺环素逆转。我们的研究结果支持这样的观点，即辣椒素注射后的神经源性炎症涉及通过产生DRR逆向激活DRG神经元。米诺环素对神经源性炎症的抑制作用与其对SGCs的抑制作用有关，SGCs可能在辣椒素注射后被激活。
Neurogenic inflammation is induced by inflammatory mediators released in peripheral tissue from primary afferent nociceptors. Our previous studies suggest that neurogenic inflammation induced by intradermal injection of capsaicin results from the enhancement of dorsal root reflexes (DRRs), which involve antidromic activation of dorsal root ganglion (DRG) neurons. Numerous studies have reported the important role of glial modulation in pain. However, it remains unclear whether glial cells participate in the process of neurogenic inflammation-induced pain. Here we tested the role of DRG satellite glial cells (SGCs) in this process in anesthetized rats by administration of a glial inhibitor, minocycline. Electrical stimuli (ES, frequency 10 Hz; duration 1 ms; strength 3 mA) were applied to the cut distal ends of the L4-5 dorsal roots. The stimuli evoked antidromic action potentials designed to mimic DRRs. Local cutaneous blood flow in the hindpaw was measured using a Doppler flow meter. Antidromic ES for 10 min evoked a significant vasodilation that could be inhibited dose-dependently by local administration of the calcitonin gene-related peptide receptor antagonist, CGRP8-37. Pretreatment with capsaicin intradermally injected into the hindpaw 2 h before the ES enhanced greatly the vasodilation evoked by antidromic ES, and this enhancement could be reversed by minocycline pretreatment. Our findings support the view that neurogenic inflammation following capsaicin injection involves antidromic activation of DRG neurons via the generation of DRRs. Inhibition of neurogenic inflammation by minocycline is suggested to be associated with its inhibitory effect on SGCs that are possibly activated following capsaicin injection.
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DOI：
10.1523/jneurosci.3795-08.2008
发表时间：
2008-12-24
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Binshtok AM;Wang H;Zimmermann K;Amaya F;Vardeh D;Shi L;Brenner GJ;Ji RR;Bean BP;Woolf CJ;Samad TA
通讯作者：
Samad TA
影响因子：
15.1
作者：
Dublin, Pavel;Hanani, Menachem
通讯作者：
Hanani, Menachem
影响因子：
3.4
作者：
LEWIN, GR;LISNEY, SJW;MENDELL, LM
通讯作者：
MENDELL, LM
影响因子：
3.8
作者：
Hagenacker, T.;Czeschik, J. C.;Buesselberg, D.
通讯作者：
Buesselberg, D.
影响因子：
7.4
作者：
Ledeboer, A;Sloane, EM;Watkins, LR
通讯作者：
Watkins, LR