Molecular cloning of cDNA for rat liver gap junction protein.

Molecular cloning of cDNA for rat liver gap junction protein.
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大鼠肝间隙连接蛋白的cDNA分子克隆。

DOI:
10.1083/jcb.103.1.123
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发表时间:
1986-07
影响因子:
7.8
通讯作者:
PAUL, DL
PAUL, DL
中科院分区:
生物学1区
文献类型:
--
作者:
PAUL, DL

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相似文献

亲和纯化的抗体针对与分离的大鼠肝脏间隙连接相关的27-kD蛋白。光镜和电镜免疫细胞化学显示,该抗原是本地化的间隙连接的细胞质表面。该抗体用于从表达载体λ gt 11中的大鼠肝脏文库中选择cDNA。选择的最大cDNA包含1,494 bp,编码的蛋白质的计算分子量为32,007道尔顿。北方印迹分析表明,大脑,肾脏和胃表达的mRNA具有相似的大小和同源性,在肝脏中表达,但心脏和透镜表达不同大小,同源性较低的mRNA。
An affinity-purified antibody directed against the 27-kD protein associated with isolated rat liver gap junctions was produced. Light and electron microscopic immunocytochemistry showed that this antigen was localized specifically to the cytoplasmic surfaces of gap junctions. The antibody was used to select cDNA from a rat liver library in the expression vector lambda gt11. The largest cDNA selected contained 1,494 bp and coded for a protein with a calculated molecular mass of 32,007 daltons. Northern blot analysis indicated that brain, kidney, and stomach express an mRNA with similar size and homology to that expressed in liver, but that heart and lens express differently sized, less homologous mRNA.
DOI: 10.1083/jcb.86.2.576
发表时间: 1980-08
期刊: The Journal of cell biology
影响因子: --
作者:
Goodenough DA;Dick JS 2nd;Lyons JE
通讯作者: Lyons JE
DOI: 10.1089/dna.1985.4.39
发表时间: 1985-01-01
期刊: DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY
影响因子: --
作者:
HELMS, C;GRAHAM, MY;OLSON, MV
通讯作者: OLSON, MV
DOI: 10.1016/0092-8674(83)90188-5
发表时间: 1983-01-01
期刊: CELL
影响因子: 64.5
作者:
GROS, DB;NICHOLSON, BJ;REVEL, JP
通讯作者: REVEL, JP
DOI: 10.1016/0022-2836(79)90391-7
发表时间: 1979-01-01
影响因子: 5.6
作者:
HENDERSON, D;EIBL, H;WEBER, K
通讯作者: WEBER, K
DOI: 10.1083/jcb.90.2.521
发表时间: 1981-08
期刊: The Journal of cell biology
影响因子: --
作者:
Fallon RF;Goodenough DA
通讯作者: Goodenough DA