Temporal dynamics of the very premature infant gut dominant microbiota.
Temporal dynamics of the very premature infant gut dominant microbiota.
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DOI：
10.1186/s12866-014-0325-0
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发表时间：
2014-12-31
期刊：
影响因子：
4.2
通讯作者：
Jumas-Bilak E
中科院分区：
文献类型：
--
作者：
Aujoulat F;Roudière L;Picaud JC;Jacquot A;Filleron A;Neveu D;Baum TP;Marchandin H;Jumas-Bilak E
早产儿肠道微生物区系由于在威胁生命的疾病的发展中可能起到的作用而越来越受到人们的关注。高通量研究的结果证实并重新引起了人们对较低分辨率的方法的兴趣，如聚合酶链式反应-时间温度凝胶电泳法(TTGE)，用于跟踪优势微生物区系动态。我们在这里报告了一项关于极早产儿肠道定植的广泛纵向研究。应用16S rDNA聚合酶链式反应(16S rDNA-PCR-TTGE)技术，对30例胎龄在30周前出生的早产儿进行1~8周常规监测，共采集粪便标本354份。结合多样性阶梯的比较和测序，可以归入50个物种级别的可操作分类单元(SLOTU)，以及对可操作分类单元(OTU)进行半定量估计。研究期间所有婴儿均检出凝固酶阴性葡萄球菌，主要为表皮葡萄球菌，且自出生以来最常见(占SLOTU总数的75.7%)。肠球菌存在于60%的婴儿中，是早产儿肠道的早期、高度代表性和持久的定殖者。肠杆菌科和梭菌属出现较晚，分别为10例(33%)和21例(70%)。我们显示，在超过四分之一的婴儿中，韦洛氏菌具有很高的代表性，并且能够持续地定植在早产儿的肠道中。厌氧菌属、水生杆菌属、芽孢杆菌属、双歧杆菌属、棒状杆菌属、微球菌属、海洋杆菌属、丙酸杆菌属、假单胞菌属、罗氏菌属、八叠球菌属、单胞菌属和链球菌属为暂时性或持久性定殖菌，每一属均存在于少数婴儿体内。尽管分辨率低，但PCR-TTGE仍然是高通量测序方法的补充，因为它允许在大量早产儿的纵向队列中跟踪肠道微生物区系中的优势细菌。我们描述了早期肠道微生物区系的发展，关于主要OTUS的功能作用，现在应该替换这些微生物区系。本文的在线版本(doi：10.1186/s12866-0140325-0)包含补充材料，授权用户可以使用。
The very-preterm infant gut microbiota is increasingly explored due to its probable role in the development of life threatening diseases. Results of high-throughput studies validate and renew the interest in approaches with lower resolution such as PCR-Temporal Temperature Gel Electrophoresis (TTGE) for the follow-up of dominant microbiota dynamics. We report here an extensive longitudinal study of gut colonization in very preterm infants. We explored by 16S rDNA-based PCR-TTGE a total of 354 stool specimens sampled during routine monitoring from the 1st to the 8th week of life in 30 very pre-term infants born before 30 weeks of gestational age. Combining comparison with a diversity ladder and sequencing allowed affiliation of 50 Species-Level Operational Taxonomic Units (SLOTUs) as well as semi-quantitative estimation of Operational Taxonomic Units (OTUs). Coagulase-negative staphylococci, mainly the Staphylococcus epidermidis, was found in all the infants during the study period and was the most represented (75.7% of the SLOTUs) from the first days of life. Enterococci, present in 60% of the infants were early, highly represented and persistent colonizers of the premature gut. Later Enterobacteriaceae and the genus Clostridium appeared and were found in 10 (33%) and 21 infants (70%), respectively. We showed a high representation of Veillonella in more than a quarter of the infants and being able to persistently colonize premature gut. The genera Anaerococcus, Aquabacterium, Bacillus, Bifidobacterium, Corynebacterium, Micrococcus, Oceanobacillus, Propionibacterium, Pseudomonas, Rothia, Sarcina, Sneathia and Streptococcus were observed as transient or persistent colonizers, each genus being found in a minority of infants. Despite low resolution, PCR-TTGE remains complementary to high-throughput sequencing-based approaches because it allows the follow-up of dominant bacteria in gut microbiota in a large longitudinal cohorts of preterm neonates. We described the development of pre-term gut microbiota that should be now replaced regarding the functional role of major OTUs. The online version of this article (doi:10.1186/s12866-014-0325-0) contains supplementary material, which is available to authorized users.
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