Generation of all-male-like sterile zebrafish by eliminating primordial germ cells at early development.
Generation of all-male-like sterile zebrafish by eliminating primordial germ cells at early development.
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DOI：
10.1038/s41598-018-20039-3
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发表时间：
2018-01-30
影响因子：
4.6
通讯作者：
Li K
中科院分区：
文献类型：
--
作者：
Zhou L;Feng Y;Wang F;Dong X;Jiang L;Liu C;Zhao Q;Li K
生产全雄鱼和不育鱼不仅可以大幅提高产量，而且对于基因组修饰物种在水产养殖中的应用至关重要。在本研究中，我们构建了一个转基因斑马鱼品系Tg（nanos 3：nfsB-mCherry-nanos 3 3 3′UTR），其中NfsB硝基还原酶仅在原始生殖细胞中表达。用MTZ处理0 ~ 2dpf（受精后天数）的转基因雌性斑马鱼胚胎，发现大于2.5dpf的胚胎生殖细胞完全消失。在20 dpf，MTZ处理的少年没有生殖细胞在他们的性腺。在100 dpf时，尽管雄性生殖腺中没有生殖细胞，只有支持细胞，但经MTX处理的成虫表现出雄性样形态，并表现出正常的交配行为。综上所述，我们的研究结果表明，在早期发育过程中的PGCs的条件消除使斑马鱼雄性样和不育。这为培育不育的全雄鱼提供了一种新的策略，有利于提高水产养殖产量，防止基因组修饰物种潜在的生态风险。
Production of all-male and sterile fish may not only substantially improve yield but also be crucial for the application of genome modified species in aquaculture. Previously, it was reported that the fish lacking primordial germ cells (PGCs) becomes infertile, and nitroreductase, an enzyme converting non-toxic metronidazole (MTZ) into toxic metabolites, induces targeted toxicity to kill the cells expressing it. In this study, we generated a transgenic zebrafish line of Tg(nanos3:nfsB-mCherry-nanos3 3′UTR) in which the NfsB nitroreductase is solely expressed in PGCs. Treating the embryos derived from the female transgenic zebrafish with MTZ from 0 through 2 dpf (days post fertilization), we found that the germ cells were completely eliminated in the ones older than 2.5 dpf. At 20 dpf, the MTZ-treated juvenile had no germ cells in their gonads. At 100 dpf, the MTZ-treated adult exhibited male-like morphology and showed normal mating behaviors although they had no germ cells but only supporting cells in their gonads. Taken together, our results demonstrated that conditional elimination of PGCs during early development make the zebrafish male-like and infertile. It may provide an alternative strategy to make sterile and all-male farmed fish that is good for increasing aquaculture yield and preventing the genome modified species from potential ecological risks.
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影响因子：
4.6
作者：
Seki S;Kusano K;Lee S;Iwasaki Y;Yagisawa M;Ishida M;Hiratsuka T;Sasado T;Naruse K;Yoshizaki G
通讯作者：
Yoshizaki G
影响因子：
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作者：
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通讯作者：
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DOI：
10.1016/j.bbrc.2016.08.156
发表时间：
2016-09-23
影响因子：
3.1
作者：
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通讯作者：
Yoshizaki, Goro
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影响因子：
9.1
作者：
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通讯作者：
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