Hormonally Regulated Double- and Single-stranded DNA-binding Complexes Involved in Mouse -Casein Gene Transcription (*)
Hormonally Regulated Double- and Single-stranded DNA-binding Complexes Involved in Mouse -Casein Gene Transcription (*)
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参与小鼠酪蛋白基因转录的激素调节双链和单链 DNA 结合复合物 (*)
DOI：
10.1074/jbc.271.15.8911
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
T. Oka
中科院分区：
文献类型：
--
作者：
H. Saito;T. Oka
在原代乳腺上皮细胞培养物中，胰岛素、催乳素和糖皮质激素的协同作用诱导252个碱基对长的小鼠β-酪蛋白基因启动子的转录。该启动子包含称为C区的区域，其在许多酪蛋白基因中具有高度保守的序列和位置。块C的突变降低了84%的催乳激素的启动子的响应。哺乳期小鼠乳腺的核提取物含有双链和单链DNA结合蛋白复合物（DS 1和SS），它们分别特异性结合C区的序列AAATTAGCATGT和CCACAA。DS 1和SS蛋白复合物分别约为400和280 kDa。每个复合物含有分子量约为120 kDa（DS 1）和80和65 kDa（SS）的DNA结合组分。脱氧胆酸干扰蛋白质-蛋白质相互作用，抑制DS 1和SS的结合活性。乳腺中DS 1和SS结合活性的最大增加分别发生在妊娠期和哺乳期。在器官培养中，表皮生长因子或催乳素与胰岛素联合可增加DS 1活性，而胰岛素、催乳素和糖皮质激素可增强SS活性。这些结果表明，多蛋白复合物结合的双链和单链DNA的C块介导的激素诱导β-酪蛋白基因转录。
Transcription of the 252-base pair-long mouse β-casein gene promoter is induced by the synergistic action of insulin, prolactin, and glucocorticoid in a primary mammary epithelial cell culture. The promoter contains a region termed block C having a highly conserved sequence and position among many casein genes. Mutation of block C reduced the response of the promoter to lactogenic hormones 84%. Nuclear extracts from lactating mouse mammary glands contained both a double-stranded and a single-stranded DNA binding protein complex (DS1 and SS), which specifically bind to the sequences AAATTAGCATGT and CCACAA of block C, respectively. The DS1 and the SS protein complexes were approximately 400 and 280 kDa, respectively. Each complex contained a DNA-binding component(s) having a molecular mass of approximately 120 kDa for DS1 and 80 and 65 kDa for SS. Deoxycholate, which interferes with the protein-protein interactions, inhibited the binding activities of DS1 and SS. The maximal increase in the binding activity of DS1 and SS in the mammary gland occurred during pregnancy and during lactation, respectively. In organ culture, the DS1 activity is increased by epidermal growth factor or prolactin in combination with insulin, whereas the SS activity is enhanced by insulin, prolactin, and glucocorticoid. These results suggest that multiprotein complexes binding to the double- and single-stranded DNA of block C mediate hormonal induction of β-casein gene transcription.
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脂肪细胞分化中脂肪素基因的控制。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wilkison,WO;Min,HY;Claffey,KP;Satterberg,BL;Spiegelman,BM
通讯作者：
Spiegelman,BM
DOI：
10.1016/s0021-9258(18)88885-8
发表时间：
1985-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
W. K. Jones;L. Yu-Lee;S. Clift;T. L. Brown;J. Rosen
通讯作者：
W. K. Jones;L. Yu-Lee;S. Clift;T. L. Brown;J. Rosen
不同生理状态下小鼠乳腺表皮生长因子受体水平。
影响因子：
4.8
作者：
Edery,M;Pang,K;Larson,L;Colosi,T;Nandi,S
通讯作者：
Nandi,S
酪蛋白多基因家族的进化：5侧翼和外显子区域的保守序列。
影响因子：
14.9
作者：
Yu-Lee,LY;Richter-Mann,L;Couch,CH;Stewart,AF;Mackinlay,AG;Rosen,JM
通讯作者：
Rosen,JM
影响因子：
10.5
作者：
Richard Sturm;T. Baumruker;B. R. Franza;W. Herr
通讯作者：
Richard Sturm;T. Baumruker;B. R. Franza;W. Herr