Immunoglobulin production in severe combined immunodeficient (SCID) mice reconstituted with human peripheral blood mononuclear cells
Immunoglobulin production in severe combined immunodeficient (SCID) mice reconstituted with human peripheral blood mononuclear cells
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用人外周血单核细胞重建严重联合免疫缺陷 (SCID) 小鼠的免疫球蛋白产生
DOI：
10.1002/eji.1830220329
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发表时间：
1992
影响因子：
5.4
通讯作者：
C. E. Edvard Smith
中科院分区：
文献类型：
--
作者：
M. Abedi;B. Christensson;K. Islam;L. Hammarström;C. E. Edvard Smith
用人外周血单核细胞重建免疫缺陷 C.B-17 scid/scid (SCID) 小鼠，并分析其体液免疫。腹腔注射后 8-12 周，大多数过继转移动物的人 IgG 血清水平为 1-4 mg/ml。用 20 × 106 PBMC 重建，而 IgM，尤其是 IgA 浓度则相当低。人 IgG、IgM 和 IgA 在 SCID 小鼠中的半衰期分别为 12 天、36 和 23 小时。此外，IgA 迅速分泌到胆汁中，表明低 IgA 浓度主要是由于高周转率造成的。小鼠血清中的 IgG 亚类分布与供体血清中的分布相似。过继转移前进行 3 Gy 照射导致重建后早期人 IgG 水平升高，而 IgM 和 IgA 浓度均受损。人类细胞转移 4 周后发现多克隆血清 Ig 模式，随后经常出现寡克隆带占优势。出乎意料的是，在 Epstein-Barr 病毒血清学阴性的供体中也发现了寡克隆条带。人类细胞被发现在腹膜腔内驻留数月。重建后两周内，在肝门附近的淋巴结构中也发现了人类细胞，随后扩散到其他淋巴器官。尚未发现人类细胞归巢至皮肤和肠道。
Immunodeficient C.B‐17 scid/scid (SCID) mice were reconstituted with human peripheral blood mononuclear cells and analyzed for humoral immunity. The majority of adoptively transferred animals had serum levels of 1–4 mg/ml of human IgG 8–12 weeks after i.p. reconstitution with 20 × 106 PBMC, whereas the IgM and especially IgA concentrations were considerably lower. The half‐lives of human IgG, IgM, and IgA in SCID mice were 12 days, 36, and 23 hours, respectively. Furthermore, IgA was rapidly secreted into bile indicating that the low IgA concentration was mainly caused by a high turnover rate. IgG subclass distribution in mouse serum was similar to that found in the donor serum. Irradiation with 3 Gy prior to adoptive transfer resulted in increased levels of human IgG early after reconstitution, whereas both IgM and IgA concentrations were impaired. A polyclonal serum Ig pattern was found 4 weeks after transfer of human cells later frequently followed by a predominance of oligoclonal bands. Unexpectedly, oligoclonal bands were also found using donors with a negative Epstein‐Barr virus serology. Human cells were found to reside in the peritoneal cavity for several months. Within 2 weeks of reconstitution, human cells were also identified in lymphoid structures in the vicinity of the liver hilus with a later spread to other lymphoid organs. Homing of human cells to skin and gut was not seen.
影响因子：
56.9
作者：
MCCUNE, JM;NAMIKAWA, R;WEISSMAN, IL
通讯作者：
WEISSMAN, IL
影响因子：
15.9
作者：
SAXON, A;MACY, E;BRAUN, J
通讯作者：
BRAUN, J