Further Extension of Lifespan by Unc-43/CaMKII and Egl-8/PLCβ Mutations in Germline-Deficient Caenorhabditis elegans.
Further Extension of Lifespan by Unc-43/CaMKII and Egl-8/PLCβ Mutations in Germline-Deficient Caenorhabditis elegans.
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胰岛素/胰岛素样生长因子1（IGF 1）信号（IIS）的减少促进了跨物种的长寿。在线虫秀丽隐杆线虫中，生殖系干细胞（GSC）的消融和保守信号传导介质unc-43/CaMKII（钙/钙调蛋白依赖性激酶II型）和egl-8/PLCβ（磷脂酶Cβ）的活性变化也增加了寿命。与IIS一样，这些途径依赖于保守的转录因子daf-16/FOXO来延长寿命，但它们在功能上如何相互作用尚不清楚。在这里，我们表明，改变unc-43/egl-8活性进一步增加了长寿的GSC缺陷蠕虫的寿命，但不是由于胰岛素/IGF 1样受体daf-2的功能突变强烈减少而长寿的蠕虫。此外，我们提供了unc-43和egl-8在较小程度上调节某些胶原基因表达的证据，据报道，这些胶原基因与这些特定的daf-2突变体蠕虫的寿命有关，但与其他形式的寿命无关。总之，这些结果为CaMKII和PLCβ信号调节C的条件和潜在机制提供了新的见解。elegans寿命。
Reduction of insulin/insulin-like growth factor 1 (IGF1) signaling (IIS) promotes longevity across species. In the nematode Caenorhabditis elegans, ablation of germline stem cells (GSCs) and activity changes of the conserved signaling mediators unc-43/CaMKII (calcium/calmodulin-dependent kinase type II) and egl-8/PLCβ (phospholipase Cβ) also increase lifespan. Like IIS, these pathways depend on the conserved transcription factor daf-16/FOXO for lifespan extension, but how they functionally interact is unknown. Here, we show that altered unc-43/egl-8 activity further increases the lifespan of long-lived GSC-deficient worms, but not of worms that are long-lived due to a strong reduction-of-function mutation in the insulin/IGF1-like receptor daf-2. Additionally, we provide evidence for unc-43 and, to a lesser extent, egl-8 modulating the expression of certain collagen genes, which were reported to be dispensable for longevity of these particular daf-2 mutant worms, but not for other forms of longevity. Together, these results provide new insights into the conditions and potential mechanisms by which CaMKII- and PLCβ-signals modulate C. elegans lifespan.
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影响因子：
48
作者：
Huber W;Carey VJ;Gentleman R;Anders S;Carlson M;Carvalho BS;Bravo HC;Davis S;Gatto L;Girke T;Gottardo R;Hahne F;Hansen KD;Irizarry RA;Lawrence M;Love MI;MacDonald J;Obenchain V;Oleś AK;Pagès H;Reyes A;Shannon P;Smyth GK;Tenenbaum D;Waldron L;Morgan M
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影响因子：
56.9
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Arantes-Oliveira, N;Apfeld, J;Kenyon, C
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Kenyon, C
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4.5
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Ghazi A;Henis-Korenblit S;Kenyon C
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Kenyon C
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12.3
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