Interactive effects of in vitro binge-like alcohol and ATP on umbilical endothelial nitric oxide synthase post-translational modifications and redox modulation.
Interactive effects of in vitro binge-like alcohol and ATP on umbilical endothelial nitric oxide synthase post-translational modifications and redox modulation.
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DOI：
10.1016/j.reprotox.2013.11.006
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发表时间：
2014-01
期刊：
影响因子：
--
通讯作者：
Ramadoss J
中科院分区：
文献类型：
--
作者：
Subramanian K;Naik VD;Sathishkumar K;Sawant OB;Washburn SE;Wu G;Yallampalli C;Saade GR;Hankins GD;Ramadoss J
酒精使生殖血管适应性调节失调。我们在此研究了慢性酗酒对脐静脉内皮一氧化氮合酶（eNOS）多位点磷酸化和相关的氧化还原开关基础（未刺激）和刺激（ATP）状态下的影响。酒精降低内皮细胞兴奋性Pser 1177 eNOS（P<0.001），而兴奋性Pser 635 eNOS主要受酒精（↓P=0.016）和ATP（↑P<0.001）的影响。酒精降低Pthr 495 eNOS水平（P=0.004），而抑制性Pser 116 eNOS在基础和刺激状态下均表现出酒精的主要作用（↑P=0.005）。总eNOS减少酒精（P=0.038）。在ATP存在下，酒精抑制ERK活性（P=0.002），而AKT表现出无酒精效应。观察到酒精对S-亚硝基谷胱甘肽还原酶（↓P=0.031）和谷胱甘肽-S-转移酶（↓P=0.027）的主要影响。注意到蛋白谷胱甘肽化增加，而注意到乙醇对GSH、GSSG、NOX 2或SOD表达没有影响。因此，酒精对与血管扩张性eNOS相关的多位点翻译后修饰和氧化还原开关的影响强调了研究酒精诱导的妊娠血管功能障碍的必要性。
Alcohol dysregulates the regulation of reproductive vascular adaptations. We herein investigated chronic binge-like alcohol effects on umbilical endothelial nitric oxide synthase (eNOS) multi-site phosphorylation and related redox switches under basal (unstimulated) and stimulated (with ATP) states. Alcohol decreased endothelial excitatory Pser1177eNOS (P<0.001), whereas excitatory Pser635eNOS exhibited a main effect of alcohol (↓P=0.016) and ATP (↑P<0.001). Alcohol decreased Pthr495eNOS (P=0.004) levels, whereas inhibitory Pser116eNOS exhibited an alcohol main effect in both basal and stimulated states (↑P=0.005). Total eNOS was reduced by alcohol (P=0.038). In presence of ATP, alcohol inhibited ERK activity (P=0.002), whereas AKT exhibited no alcohol effect. Alcohol main effect on S-nitroso-glutathione reductase (↓P=0.031) and glutathione-S-transferase (↓P=0.027) were noted. Increased protein glutathiolation was noted, whereas no alcohol effect on GSH, GSSG, NOX2 or SOD expression was noted. Thus, alcohol effects on multi-site post-translational modifications and redox switches related to vasodilatory eNOS underscore the necessity for investigating alcohol-induced gestational vascular dysfunction.
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影响因子：
64.8
作者：
Chen, Chun-An;Wang, Tse-Yao;Varadharaj, Saradhadevi;Reyes, Levy A.;Hemann, Craig;Talukder, M. A. Hassan;Chen, Yeong-Renn;Druhan, Lawrence J.;Zweier, Jay L.
通讯作者：
Zweier, Jay L.
DOI：
10.1111/j.1530-0277.1997.tb04247.x
发表时间：
1997-09-01
影响因子：
3.2
作者：
Eysseric, H;Gonthier, B;Barret, L
通讯作者：
Barret, L
DOI：
10.1097/01.alc.0000130811.92457.0d
发表时间：
2004-07-01
影响因子：
3.2
作者：
Kuhlmann, CRW;Li, F;Wiecha, J
通讯作者：
Wiecha, J
影响因子：
64.8
作者：
Dimmeler, S;Fleming, I;Zeiher, AM
通讯作者：
Zeiher, AM
影响因子：
4.8
作者：
Boo, YC;Sorescu, G;Jo, HJ
通讯作者：
Jo, HJ