Predictive value of TRPV2 expression from peripheral blood mononuclear cells on the early recurrence of atrial fibrillation after radiofrequency catheter ablation.
Predictive value of TRPV2 expression from peripheral blood mononuclear cells on the early recurrence of atrial fibrillation after radiofrequency catheter ablation.
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外周血单个核细胞TRPV2表达对射频消融术后房颤早期复发的预测价值
DOI：
10.1186/s12872-022-02992-0
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发表时间：
2022-12-13
影响因子：
2.1
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中科院分区：
文献类型：
--
作者：
最近的研究表明，瞬时受体电位香草酸2（TRPV 2）通道仅在房颤（AF）患者中上调，我们的目的是研究TRPV 2 mRNA在有/无早期复发房颤（ERAF）患者外周血单个核细胞（PBMC）中的表达水平，在射频导管消融术（RFCA）后，并找到一个可靠的预测ERAF。65例成功行射频消融术的房颤患者，在术后3个月内根据ERAF分为两组。在RFCA之前和之后通过Ficoll梯度离心从全血中分离PBMC。进行基因集富集分析以评估TRPV通道表达水平，并使用京都基因和基因组百科全书（KEGG）作图进行途径富集分析。RFCA前两组间TRPV 2 mRNA表达水平无显著差异，而RFCA后无ERAF组TRPV 2 mRNA表达较ERAF组明显降低。通过受试者操作特征和Kaplan-Meier生存曲线分析，首次证实TRPV 2表达数量是一个独立的预测因子。需要指出的是，上述结果仅用于预测ERAF，根据现有数据对房颤晚期复发无预测意义。此外，ERF与P波离散度呈负相关。KEGG图谱进一步聚类了41条通路，揭示了“环鸟苷一磷酸-蛋白激酶G信号通路”的显著富集。我们首先假设RFCA后无ERAF的患者外周TRPV 2表达下调。因此，TRPV 2可能代表成功射频消融后早期阶段的新预测因子。在线版本包含补充材料，可通过10.1186/s12872-022-02992-0获得。
Recent study has shown that the transient receptor potential vanilloid 2 (TRPV2) channel was exclusively upregulated in patients with atrial fibrillation (AF), and that this overexpression might be detrimental for occurrence and maintenance of AF. We aimed to characterize the expression levels of TRPV2 mRNA in peripheral blood mononuclear cells (PBMCs) with/without early recurrence of atrial fibrillation (ERAF) after radiofrequency catheter ablation (RFCA), and to find a reliable predictor for ERAF. 65 patients of AF, who underwent RFCA successfully, then divided into two groups according to ERAF during following 3 months. PBMCs were isolated from whole blood by Ficoll gradient centrifugation before and after RFCA. Gene set enrichment analysis was performed to evaluate TRPV channels expression levels and Kyoto Encyclopedia of Genes and Genomes (KEGG) mapping was used for pathway enrichment analysis. There was no significant difference in the TRPV2 mRNA expression level between the two groups before RFCA, while without ERAF group of TRPV2 expression was markedly reduced compared to ERAF group after RFCA. Moreover, the number of TRPV2 expression was confirmed as an independent predictor for the first time through receiver operating characteristic and Kaplan–Meier survival curve analysis. It should be pointed out that the above results were only used to predict ERAF, and have no predictive significance for late recurrence of atrial fibrillation according to the current data. Additionally, ERAF was inversely correlated with P wave dispersion. KEGG mapping further clustered 41 pathways, revealing that ‘‘cyclic guanosine monophosphate-protein kinase G signaling pathway’’ was significantly enriched. We firstly assume that downregulated expression of peripheral TRPV2 appear in patients without ERAF after RFCA. TRPV2 may thus represent a novel predictor of early phase after successful radiofrequency ablation. The online version contains supplementary material available at 10.1186/s12872-022-02992-0.
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影响因子：
3.7
作者：
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