Gene Expression of Transient Receptor Potential Channels in Peripheral Blood Mononuclear Cells of Inflammatory Bowel Disease Patients.
Gene Expression of Transient Receptor Potential Channels in Peripheral Blood Mononuclear Cells of Inflammatory Bowel Disease Patients.
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DOI：
10.3390/jcm9082643
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发表时间：
2020-08-14
影响因子：
3.9
通讯作者：
Torimura T
中科院分区：
文献类型：
--
作者：
Morita T;Mitsuyama K;Yamasaki H;Mori A;Yoshimura T;Araki T;Morita M;Tsuruta K;Yamasaki S;Kuwaki K;Yoshioka S;Takedatsu H;Torimura T
我们检测了炎症性肠病（IBD）患者外周血单个核细胞（PBMC）中瞬时受体电位（TRP）通道的表达谱。从41名溃疡性结肠炎（UC）患者、34名克罗恩病（CD）患者和30名正常受试者获得PBMC。采用实时定量聚合酶链反应测定TRP通道的mRNA水平，并进行与疾病等级以及实验室参数的相关性检验。与对照组相比，UC和CD患者PBMCs中TRPV2和TRPC1 mRNA表达降低，TRPM2 mRNA表达升高。CD患者TRPV3 mRNA表达降低，TRPV4 mRNA表达升高。CD组TRPC 6 mRNA表达高于UC组。在UC和CD患者中，TRPV 2 mRNA的表达与疾病活动性呈负相关，而TRPM 4 mRNA的表达仅在UC患者中与疾病活动性呈负相关。IBD患者PBMCs中TRP通道成员的mRNA表达水平不同，可能在IBD的进展中起重要作用。
We examined the expression profile of transient receptor potential (TRP) channels in peripheral blood mononuclear cells (PBMCs) from patients with inflammatory bowel disease (IBD). PBMCs were obtained from 41 ulcerative colitis (UC) patients, 34 Crohn’s disease (CD) patients, and 30 normal subjects. mRNA levels of TRP channels were measured using the quantitative real-time polymerase chain reaction, and correlation tests with disease ranking, as well as laboratory parameters, were performed. Compared with controls, TRPV2 and TRPC1 mRNA expression was lower, while that of TRPM2, was higher in PBMCs of UC and CD patients. Moreover, TRPV3 mRNA expression was lower, while that of TRPV4 was higher in CD patients. TRPC6 mRNA expression was higher in patients with CD than in patients with UC. There was also a tendency for the expression of TRPV2 mRNA to be negatively correlated with disease activity in patients with UC and CD, while that of TRPM4 mRNA was negatively correlated with disease activity only in patients with UC. PBMCs from patients with IBD exhibited varying mRNA expression levels of TRP channel members, which may play an important role in the progression of IBD.
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影响因子：
30.5
作者：
通讯作者：
--
影响因子：
64.8
作者：
Gerbe F;Sidot E;Smyth DJ;Ohmoto M;Matsumoto I;Dardalhon V;Cesses P;Garnier L;Pouzolles M;Brulin B;Bruschi M;Harcus Y;Zimmermann VS;Taylor N;Maizels RM;Jay P
通讯作者：
Jay P
影响因子：
56.9
作者：
Launay, P;Cheng, H;Kinet, JP
通讯作者：
Kinet, JP
影响因子：
3.7
作者：
Santos FICD;Marini N;Santos RSD;Hoffman BSF;Alves-Ferreira M;de Oliveira AC
通讯作者：
de Oliveira AC
影响因子：
64.8
作者：
Khor, Bernard;Gardet, Agnes;Xavier, Ramnik J.
通讯作者：
Xavier, Ramnik J.